المستودع الأكاديمي جامعة المدينة

Empirical validation of a two-state kinetic model for the B-Z transition of double-stranded poly[d(G-m 5 C)]

أعرض تسجيلة المادة بشكل مبسط

dc.contributor Fundación Ramón Areces
dc.creator Fuertes, Miguel Ángel
dc.creator González, Víctor Manuel
dc.creator Pérez, José Manuel
dc.creator Alonso, Carlos
dc.date 2007-12-20T15:55:15Z
dc.date 2007-12-20T15:55:15Z
dc.date 1999-12
dc.date.accessioned 2017-01-31T00:59:27Z
dc.date.available 2017-01-31T00:59:27Z
dc.identifier Journal of Biological Inorganic Chemistry 4 (6): 759-765
dc.identifier http://hdl.handle.net/2027.42/42325
dc.identifier 0949-8257
dc.identifier http://hdl.handle.net/10261/2559
dc.identifier 10.1007/s007750050348
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/2027
dc.description A formula based on a first-order kinetic equation is derived to evaluate the rate constant of the B-Z transition of a synthetic double-stranded poly[d(G-m5C)] in terms of the salt concentration, the absolute temperature, and the cooperativity index. The validity of the formula was tested using circular dichroism spectroscopy after variation of the type of salt (NaCl, MgCl2), the salt concentration, and the temperature of the polynucleotide solution. A consequence of the proposed function is that in conditions of high salt there is a predictable salt threshold which determines the particular molecular mechanism of the B-Z transition. The paper also describes the way in which this threshold level is temperature dependent. A detailed comparison of our data with the experimental data found by other authors is given. The function agrees quantitatively with the experiments and explains the contrasting results found in the literature about the influence in the B-Z transition of both the temperature and the polymer size.
dc.description This work was supported by grant BIO96-0405. An institutional grant from the Fundación Ramón Areces is also acknowledged.
dc.description Peer reviewed
dc.language eng
dc.publisher Springer
dc.rights openAccess
dc.subject Circular dichroism spectroscopy
dc.subject Synthetic polynucleotide
dc.subject Salt dependence
dc.subject Temperature dependence
dc.subject Left-handed DNA
dc.title Empirical validation of a two-state kinetic model for the B-Z transition of double-stranded poly[d(G-m 5 C)]
dc.type Artículo


الملفات في هذه المادة

الملفات الحجم الصيغة عرض

لا توجد أي ملفات مرتبطة بهذه المادة.

هذه المادة تبدو في المجموعات التالية:

أعرض تسجيلة المادة بشكل مبسط