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Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa

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dc.creator Gagliardi Rachel Fatima
dc.creator Pacheco Georgia Pereira
dc.creator Oliveira Carlos Alberto
dc.creator Carneiro Leonardo Alves
dc.creator Valls José Francisco Montenegro
dc.creator Vieira Maria Lucia Carneiro
dc.creator Mansur Elisabeth
dc.date 2004
dc.date.accessioned 2013-06-01T10:16:20Z
dc.date.available 2013-06-01T10:16:20Z
dc.date.issued 2013-06-01
dc.identifier http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000200015
dc.identifier http://www.doaj.org/doaj?func=openurl&genre=article&issn=0100204X&date=2004&volume=39&issue=2&spage=197
dc.identifier.uri http://koha.mediu.edu.my:8181/jspui/handle/123456789/8115
dc.description In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.
dc.publisher Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
dc.source Pesquisa Agropecuária Brasileira
dc.subject germplasm conservation
dc.subject micropropagation
dc.subject molecular characterization
dc.subject genetic stability
dc.title Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa


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