| dc.creator |
Nibedita Gupta |
|
| dc.creator |
Fee Schmitt |
|
| dc.creator |
Sina Grebhardt |
|
| dc.creator |
Doris Mayer |
|
| dc.date |
2011 |
|
| dc.date.accessioned |
2013-05-30T13:10:42Z |
|
| dc.date.available |
2013-05-30T13:10:42Z |
|
| dc.date.issued |
2013-05-30 |
|
| dc.identifier |
http://www.mdpi.com/2072-6694/3/3/2990/ |
|
| dc.identifier |
http://www.doaj.org/doaj?func=openurl&genre=article&issn=20726694&date=2011&volume=3&issue=3&spage=2990 |
|
| dc.identifier.uri |
http://koha.mediu.edu.my:8181/jspui/handle/123456789/5642 |
|
| dc.description |
Estrogen receptor-alpha (ERα) is a key factor in the development of breast cancer in humans. The expression and activity of ERα is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between β-catenin and ERα in human breast cancer cells. Knockdown of β-catenin by RNAi resulted in significant reduction of ERα mRNA and/or protein levels in MCF-7, T-47D, and BT-474 breast cancer cells and in significant reduction of estradiol-induced expression of the ERα target genes pS2 and GREB1. In addition β-catenin silencing resulted in significant decrease of growth of MCF-7 cells both in the absence and presence of estradiol. β-catenin and ERα could not be co-immunoprecipitated by ERα antibodies from lysates of E2-treated or untreated cells suggesting lack of direct physical interaction. It is concluded that β-catenin is a positive regulator of ERα mRNA and protein expression. |
|
| dc.language |
eng |
|
| dc.publisher |
Molecular Diversity Preservation International |
|
| dc.source |
Cancers |
|
| dc.subject |
β-catenin |
|
| dc.subject |
estrogen receptor |
|
| dc.subject |
cross-talk |
|
| dc.subject |
gene expression |
|
| dc.subject |
transcriptional activity |
|
| dc.subject |
breast cancer cell |
|
| dc.title |
β-Catenin Is a Positive Regulator of Estrogen Receptor-α Function in Breast Cancer Cells |
|