| dc.creator |
Rim Chatter |
|
| dc.creator |
Rym Ben Othman |
|
| dc.creator |
Sameh Rabhi |
|
| dc.creator |
Maria Kladi |
|
| dc.creator |
Safa Tarhouni |
|
| dc.creator |
Constantinos Vagias |
|
| dc.creator |
Vassilios Roussis |
|
| dc.creator |
Lamia Guizani-Tabbane |
|
| dc.creator |
Riadh Kharrat |
|
| dc.date |
2011 |
|
| dc.date.accessioned |
2013-05-30T13:09:09Z |
|
| dc.date.available |
2013-05-30T13:09:09Z |
|
| dc.date.issued |
2013-05-30 |
|
| dc.identifier |
http://www.mdpi.com/1660-3397/9/7/1293/ |
|
| dc.identifier |
http://www.doaj.org/doaj?func=openurl&genre=article&issn=16603397&date=2011&volume=9&issue=7&spage=1293 |
|
| dc.identifier.uri |
http://koha.mediu.edu.my:8181/jspui/handle/123456789/5627 |
|
| dc.description |
Neorogioltriol is a tricyclic brominated diterpenoid isolated from the organic extract of the red algae Laurencia glandulifera. In the present study, the anti-inflammatory effects of neorogioltriol were evaluated both in vivo using carrageenan-induced paw edema and in vitro on lipopolysaccharide (LPS)-treated Raw264.7 macrophages. The in vivo study demonstrated that the administration of 1 mg/kg of neorogioltriol resulted in the significant reduction of carregeenan-induced rat edema. In vitro, our results show that neorogioltriol treatment decreased the luciferase activity in LPS-stimulated Raw264.7 cells, stably transfected with the NF-κB-dependent luciferase reporter. This effect on NF-κB activation is not mediated through MAPK pathways. The inhibition of NF-κB activity correlates with decreased levels of LPS-induced tumor necrosis factor-alpha (TNFα) present in neorogioltriol treated supernatant cell culture. Further analyses indicated that this product also significantly inhibited the release of nitric oxide and the expression of cyclooxygenase-2 (COX-2) in LPS-stimulated Raw264.7 cells. These latter effects could only be observed for neorogioltriol concentrations below 62.5 µM. To our knowledge, this is the first report describing a molecule derived from Laurencia glandulifera with anti-inflammatory activity both in vivo and in vitro. The effect demonstrated in vitro may be explained by the inhibition of the LPS-induced NF-κB activation and TNFα production. NO release and COX-2 expression may reinforce this effect. |
|
| dc.language |
eng |
|
| dc.publisher |
Molecular Diversity Preservation International |
|
| dc.source |
Marine Drugs |
|
| dc.subject |
anti-inflammatory |
|
| dc.subject |
neorogioltriol |
|
| dc.subject |
Laurencia |
|
| dc.subject |
TNFα |
|
| dc.subject |
NF-κB |
|
| dc.subject |
NO |
|
| dc.subject |
COX-2 |
|
| dc.subject |
carrageenan |
|
| dc.title |
In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera |
|