The objective of this experiment was to determine the effect of a one course training programon some Biochemical and Immunological indexes in active girls.12 students voluntarily participated in the present study and randomly assigned to one of twogroups: experimental and control. Experimental group performed two peaks training programand control group did not perform any physical activity. Venous blood samples were obtainedfor analysis.Data were analyzed by using ANOVA test; independent t-test.The result indicatedthat value of AST dramatically increased than at the resting level. Also the value of ALT levelsafter 24 hours from second and sixth days of protocols; significantly were elevated;but GGT;neutrophil and lymphocyte had not significant changes. The result indicated that value oflymphocyte had increase and value of neutrophil decrease than control group.So; the continue of the exercise program before full recovery may increase infection risk andhepatic cell damage.