The purpose of this study was to investigate the effect of one session short-time intensivephysical activity on the some parameters of clotting system and CPK; LDH enzyme in theathlete and non-athlete female. This research was performed through Quasi-experimentalmethod. Statistical sample included athlete and 10 non-athlete female students from ALzahraUniversity who were selected randomly. Physical activity consisted of performance of theanaerobic RAST test with maximal effort or 85%-95% subjects heart rate reserved. At the enddata was analyzed through mixed factorial and multivariate test. The finding of the studyshowed: A significant increase (p<0.05) in ceratin phosphokinaz and lactate dehydrogenize afterexercise in the both of group was seen. there was no significant difference between athlete andnon-athlete subjects; although increase in the lactate dehydrogenize was higher in the nonathletethan athlete subjects and increase in the ceratin phosphokinaz was higher in the athletethan non-athlete subjects and a significant increase (p<0.05) in platelet; fibrinogen; calcium andno significant increase in MPV; protrombin time; tromboplastin time after exercise in the bothof group was seen.. Lastly since increase in most of these variables is considered cardiovascularrisk factor; this increment can be a threat for people involved in vigorous activities andespecially for in-athlete individuals who have shown more increase in these variables.