A formulation containing agents affecting the non-inflammatory like tretinoin as well as the inflammatory lesions of acne like clindamycin at the same time is preferable due to their high efficacy and shortening of the duration of treatment. Liposomes can improve the therapeutic effect of drugs and decrease the adverse effects. Therefore, liposomes containing clindamycin (Lip-CL), liposomes containing tretinoin (Lip-TRT) and liposomes loaded with both tretinoin and clindamycin (Lip-CL-TRT) were prepared and characterized. Lip-TRT were prepared by solvent evaporation method whereas Lip-CL and Lip-CL-TRT were prepared by dehydration-rehydration method. The morphologic, mean size and drug encapsulation efficiency were evaluated. Also, the amount of drug which was passed through or retained inside the skin was determined by Franz cell diffusion method and compared with the TRT cream. The particles of the liposomes were obtained in submicron size. The encapsulation efficiency (EE) of TRT and CL were high in the liposomal formulations. The retention of TRT and CL inside the skin from the Lip-CL-TRT were obtained more than 80%. Generally the results of the present study showed that it is possible to select liposomes as drug carrier for both CL and TRT.