Twenty juvemile crucian carp were set to two groups(exercise trained group and control group), and all fish were fed once daily at 25℃.Exercise-acclimated fish underwent two weeks exhaustive chasing training twice daily. Then the exess post-exercise oxygen consumption(EPOC) was measured after a about of exhaustive chasing in both the exercise-acclimated group and control-acclimated group.The lactic acid content in muscle and liver, the activity of lactate dehydrogenase(LDH)and citrate synthase (CS)in muscle were also measured 8 hours after exercise. The results revealed that the EPOC magnitude of the exercise-trained group(166.2±7.2)mg·kg-1 was significantly larger than that of the control group (108.0±6.2)mg·kg-1 while the content of muscle lactic acid in the exercise-trained group(17.4±0.8)umol·g-1 was significantly lower than that in the control group (25.4±1.7) umol·g-1(p<0.05).Exhaustive chasing training had no significant effect on the either content of liver lactic activities of muscle LdH and CS. The results suggested that exhaustive chasing training can significantly enhance the anaerobic metabolic capacity for juvenile crucian carp and the elevation of the anaerobic metabolic capacity may be associated with the enhanced clearance rate of lactic acid.