| dc.creator |
M. Dolores Blanco |
|
| dc.creator |
Sandra Guerrero |
|
| dc.creator |
Marta Benito |
|
| dc.creator |
Ana Fernández |
|
| dc.creator |
César Teijón |
|
| dc.creator |
Rosa Olmo |
|
| dc.creator |
Issa Katime |
|
| dc.creator |
José M. Teijón |
|
| dc.date |
2011 |
|
| dc.date.accessioned |
2013-05-30T11:48:09Z |
|
| dc.date.available |
2013-05-30T11:48:09Z |
|
| dc.date.issued |
2013-05-30 |
|
| dc.identifier |
http://www.mdpi.com/2073-4360/3/3/1107/ |
|
| dc.identifier |
http://www.doaj.org/doaj?func=openurl&genre=article&issn=20734360&date=2011&volume=3&issue=3&spage=1107 |
|
| dc.identifier.uri |
http://koha.mediu.edu.my:8181/jspui/handle/123456789/4824 |
|
| dc.description |
Folate-targeted poly[(p-nitrophenyl acrylate)-co-(N-isopropylacrylamide)] nanohydrogel (F-SubMG) was loaded with 5-fluorouracil (5-FU) to obtain low (16.3 ± 1.9 μg 5-FU/mg F-SubMG) and high (46.8 ± 3.8 μg 5-FU/mg F-SubMG) load 5-FU-loaded F-SubMGs. The complete in vitro drug release took place in 8 h. The cytotoxicity of unloaded F-SubMGs in MCF7 and HeLa cells was low; although it increased for high F-SubMG concentration. The administration of 10 μM 5-FU by 5-FU-loaded F-SubMGs was effective on both cellular types. Cell uptake of F-SubMGs took place in both cell types, but it was higher in HeLa cells because they are folate receptor positive. After subcutaneous administration (28 mg 5-FU/kg b.w.) in Wistar rats, F-SubMGs were detected at the site of injection under the skin. Histological studies indicated that the F-SubMGs were surrounded by connective tissue, without any signs of rejections, even 60 days after injection. Pharmacokinetic study showed an increase in MRT (mean residence time) of 5-FU when the drug was administered by drug-loaded F-SubMGs. |
|
| dc.language |
eng |
|
| dc.publisher |
Molecular Diversity Preservation International |
|
| dc.source |
Polymers |
|
| dc.subject |
drug delivery |
|
| dc.subject |
folate-conjugate nanogel |
|
| dc.subject |
5-fluorouracil |
|
| dc.subject |
culture cells |
|
| dc.subject |
pharmacokinetic |
|
| dc.title |
In Vitro and In Vivo Evaluation of a Folate-Targeted Copolymeric Submicrohydrogel Based on N-Isopropylacrylamide as 5-Fluorouracil Delivery System |
|