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Comparison of ELISA and Three Rapid HCG Dipsticks in Diagnosis of Premature Rupture of Membranes

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dc.creator N Kariman
dc.creator M Hedayati
dc.creator Z Taheri
dc.creator M Fallahian
dc.creator S Salehpoor
dc.creator SH Alavi Majd
dc.date 2011
dc.date.accessioned 2013-05-30T11:35:59Z
dc.date.available 2013-05-30T11:35:59Z
dc.date.issued 2013-05-30
dc.identifier www.ircmj.com
dc.identifier http://www.doaj.org/doaj?func=openurl&genre=article&issn=15614395&date=2011&volume=13&issue=6&spage=415
dc.identifier.uri http://koha.mediu.edu.my:8181/jspui/handle/123456789/4702
dc.description Background: The importance of accurate diagnosis of premature rupture of membranes (PROM) is quite apparentwhile trying to diminish false negative or positive results as much as possible. This study compares Enzyme-Linked Immunosorbent Assay (ELISA) and three rapid human chorionic gonadothropin (HCG) dipsticks in diagnosisof premature rupture of membranes.Methods: During 2008-2009, 181 pregnant women with single pregnancy from 14 to 41 weeks of gestation whoreferred to Ayatollah Taleghani Hospital in Tehran, Iran were divided into two groups, 91 patients with PROM and90 controls with matched gestational weeks. All patients underwent speculum examination for cervicovaginalwashing fluid, HCG three rapid tests and ELISA.Results: The HCG concentration of vaginal fluid was significantly different between the two groups. Using receiver operatingcharacteristic (ROC) curve and determining the threshold as 19 mIU/mL for HCG by ELISA method, the sensitivitywas 94.5%; specificity, 91%; positive predictive value, 91.5%; negative predicted value, 94.2% and accuracy was 92.2%.In rapid diagnostic test, the most sensitivity was for ACON and the most specificity for DIMA. Comparing the four methods,DIMA strip showed the highest accuracy and the highest value in early diagnosis of ROM.Conclusion: The reliability of three rapid diagnostic tests in diagnosis of ROM in cervicovaginal discharge wasacceptable.
dc.publisher Iranian Hospital, Dubai
dc.source Iranian Red Crescent Medical Journal
dc.subject Premature rupture of membranes
dc.subject Human chorionic gonadotropin
dc.subject Cervicovaginal discharge
dc.subject Rapid test strip
dc.subject ELISA
dc.title Comparison of ELISA and Three Rapid HCG Dipsticks in Diagnosis of Premature Rupture of Membranes


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