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Lipid peroxidation by Pseudomonas aeruginosa in the pathogenesis of nosocomial sepsis.

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dc.creator Giamarellos-Bourboulis E
dc.creator Skiathitis S
dc.creator Dionyssiou-Asteriou A
dc.creator Hatziantoniou S
dc.creator Demetzos K
dc.creator Dontas I
dc.creator Papaioannou G
dc.creator Karatzas G
dc.creator Helen G
dc.date 2003
dc.date.accessioned 2013-05-30T11:08:42Z
dc.date.available 2013-05-30T11:08:42Z
dc.date.issued 2013-05-30
dc.identifier http://www.jpgmonline.com/article.asp?issn=0022-3859;year=2003;volume=49;issue=1;spage=11;epage=6;aulast=Giamarellos-Bourboulis
dc.identifier http://www.doaj.org/doaj?func=openurl&genre=article&issn=00223859&date=2003&volume=49&issue=1&spage=11
dc.identifier.uri http://koha.mediu.edu.my:8181/jspui/handle/123456789/4386
dc.description BACKGROUND: To study whether Pseudomonas aeruginosa may directly trigger peroxidation of polyunsaturated fatty acids, since lipid peroxidation is a mechanism involved in the pathogenesis of sepsis. METHODS: Gamma-linolenic acid (GLA) was administered intravenously at a dose of 25mg/kg in an infusion time of 10 minutes to seven male rabbits. Blood samples were collected from the hepatic veins and from the carotid artery at regular time intervals. One clinical isolate was ex vivo incubated with the serum derived from the latter samples and concentrations of malondialdehyde (MDA) were determined during incubation in the growth medium by the thiobarbiturate assay. RESULTS: Elevated concentrations of MDA compared to their basal levels were found over the first three hours of incubation in the presence of samples collected 30 to 60 minutes after the end of the infusion of GLA. After infusion of GLA concentrations of arachidonic acid in the serum increased to concentrations comparable to those detected in sepsis. CONCLUSION: Direct triggering of lipid peroxidation by nosocomial isolates might be proposed as a pathogenetic mechanism of sepsis.
dc.publisher Medknow Publications
dc.source Journal of Postgraduate Medicine
dc.subject Animal
dc.subject Arachidonic Acid
dc.subject blood
dc.subject Cross Infection
dc.subject blood
dc.subject microbiology
dc.subject Lipid Peroxidation
dc.subject Male
dc.subject Malondialdehyde
dc.subject blood
dc.subject Pseudomonas aeruginosa
dc.subject metabolism
dc.subject pathogenicity
dc.subject Rabbits
dc.subject gamma-Linolenic Acid
dc.subject administration & dosage
dc.title Lipid peroxidation by Pseudomonas aeruginosa in the pathogenesis of nosocomial sepsis.

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