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Cholesterol oxidase interference on the emergence and viability of cotton boll weevil larvae

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dc.creator Santos Roseane Cavalcanti
dc.creator Monnerat Rose Gomes
dc.creator Sá Maria de Fátima Grossi de
dc.creator Cordeiro Célia Maria Torres
dc.creator Gomes Ana Cristina
dc.creator Gander Eugen Silvano
dc.date 2002
dc.date.accessioned 2013-05-30T05:46:45Z
dc.date.available 2013-05-30T05:46:45Z
dc.date.issued 2013-05-30
dc.identifier http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002001100002
dc.identifier http://www.doaj.org/doaj?func=openurl&genre=article&issn=0100204X&date=2002&volume=37&issue=11&spage=1525
dc.identifier.uri http://koha.mediu.edu.my:8181/jspui/handle/123456789/3915
dc.description The aim of this work was to evaluate the influence of the enzyme cholesterol oxidase (Coase) on emergence and viability of larvae of the cotton boll weevil (Anthonomus grandis Boheman, 1843). A series of bioassays was performed with eggs and neonate larvae exposed to different enzyme concentrations in artificial diet. Larval survival was affected at all enzyme concentrations tested, and the six-day LD50 was 53 mug/mL (CI 95%: 43-59). Coase also interfered with hatching of larvae after eggs were floated for 15 min in Coase solution at different concentrations. Observations at the light and electronic microscopic level of midguts from larvae fed on artificial diet containing 53 mug/mL of Coase and collected at six days revealed highly vacuolated regions in the epithelial cells as well as partial degradation of the basal membrane and microvilli.
dc.publisher Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
dc.source Pesquisa Agropecuária Brasileira
dc.subject Anthonomus grandis
dc.subject Insecta
dc.subject animal tissues
dc.subject epithelium
dc.subject intestinal histology
dc.title Cholesterol oxidase interference on the emergence and viability of cotton boll weevil larvae


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