المستودع الأكاديمي جامعة المدينة

Isolation of a cotton NADP(H) oxidase homologue induced by drought stress

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dc.creator NEPOMUCENO ALEXANDRE LIMA
dc.creator STEWART JAMES MCD
dc.creator OOSTERHUIS DERRICK
dc.creator TURLEY RICKIE
dc.creator NEUMAIER MORMAN
dc.creator FARIAS JOSÉ RENATO BOUÇAS
dc.date 2000
dc.date.accessioned 2013-05-30T01:19:59Z
dc.date.available 2013-05-30T01:19:59Z
dc.date.issued 2013-05-30
dc.identifier http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000700015
dc.identifier http://www.doaj.org/doaj?func=openurl&genre=article&issn=0100204X&date=2000&volume=35&issue=7&spage=1407
dc.identifier.uri http://koha.mediu.edu.my:8181/jspui/handle/123456789/3303
dc.description The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription.
dc.publisher Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
dc.source Pesquisa Agropecuária Brasileira
dc.subject messenger RNA
dc.subject gene expression
dc.subject genetic transformation
dc.subject water deficit
dc.title Isolation of a cotton NADP(H) oxidase homologue induced by drought stress


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