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Establishment of an in vitro system for studies on the induced resistance of cotton to Xanthomonas campestris pv. malvacearum

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dc.creator KOBAYASHI ADILSON KENJI
dc.creator VIEIRA LUIZ GONZAGA ESTEVES
dc.date 2000
dc.date.accessioned 2013-05-30T00:38:15Z
dc.date.available 2013-05-30T00:38:15Z
dc.date.issued 2013-05-30
dc.identifier http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000400007
dc.identifier http://www.doaj.org/doaj?func=openurl&genre=article&issn=0100204X&date=2000&volume=35&issue=4&spage=719
dc.identifier.uri http://koha.mediu.edu.my:8181/jspui/handle/123456789/3137
dc.description An in vitro system for studying the resistance response of cotton (Gossypium hirsutum L.) to Xanthomonas campestris pv. malvacearum was investigated. Cell suspension cultures, established from hypocotyl-derived callus of cotton cultivar 101-102B, were treated with bacterial extracellular polysaccharides (EPS) extracted from the incompatible race 18 of X. campestris pv. malvacearum. EPS at 600 mug/mL caused pronounced darkening of the suspension cultures, as indicative of cell death, 48 hours after incubation. Protein electrophoresis analysis of the time course of EPS-treated cells showed differential accumulation of several protein bands after 12-24 hours. The time course of protein accumulation and cell death was consistent with an elicitor-mediated hypersensitive response.
dc.publisher Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
dc.source Pesquisa Agropecuária Brasileira
dc.subject Gossypium hirsutum
dc.subject bacterial blight
dc.subject extracellular polysaccharides
dc.subject cell suspension culture
dc.subject hypersensitive response
dc.title Establishment of an in vitro system for studies on the induced resistance of cotton to Xanthomonas campestris pv. malvacearum


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