The objective of this study was to estimate the optimum plots size for experiments with annatto. The uniformity assay consisted of 12 rows with 12 plants in each row. The variety Bico de Pato was used, planted in 5 x 4 m spacing and evaluated at 5 years of age. Three methods were used: maximum curvature, modified maximum curvature and the comparison of variances. The plot size estimate varied according to the methodology used and the characteristic analyzed. The adequate plot size was found to be 107.2 m² (5 plants) using the modified maximum curvature method, which resulted in more precise estimates , taking into consideration that the ideal plot should facilitate the efficient evaluation of all characteristics analyzed in this experiment.