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An imperfect heat shock element and different upstream sequences are required for the seed-specific expression of a small heat shock protein gene.

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dc.contributor Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
dc.contributor Junta de Andalucía
dc.creator Carranco, Raúl
dc.creator Almoguera, Concepción
dc.creator Jordano, Juan
dc.date 1999-11
dc.date.accessioned 2017-01-31T01:01:27Z
dc.date.available 2017-01-31T01:01:27Z
dc.identifier Plant Physiology 121: 723–730 (1999)
dc.identifier 1532-2548
dc.identifier 10261/3435
dc.identifier 10.1104/pp.121.3.723
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/122886
dc.description Chimeric constructs containing the promoter and upstream sequences of Ha hsp17.6 G1, a small heat shock protein gene, reproduced in transgenic tobacco (Nicotiana tabacum) its unique seed-specific expression patterns previously reported in sunflower. These constructs did not respond to heat shock, but were expressed without exogenous stress during late zygotic embryogenesis coincident with seed desiccation. Site-directed mutagenesis of its distal and imperfect heat shock element strongly impaired in vitro heat shock transcription factor binding and transgene expression in seeds. Deletion analyses of upstream sequences indicated the contribution of additional cis-acting elements with either positive or negative effects on transgene expression. These results show differences in the transcriptional activation through the heat shock element of small heat shock protein gene promoters in seeds compared with the heat shock response. In addition, they suggest that heat shock transcription factors and other distinct trans-acting factors cooperate in the regulation of Ha hsp17.6 G1 during seed desiccation.
dc.description This work was funded by the Spanish Comisión Interministerial de Ciencia y Tecnología (grant no. BIO96–0474) and by Junta de Andalucía (grant no. CVI148).
dc.description Peer reviewed
dc.language eng
dc.publisher American Society of Plant Biologists
dc.relation http://dx.doi.org/10.1104/pp.121.3.723
dc.rights closedAccess
dc.subject Heat shock element
dc.subject Upstream sequences
dc.subject Seed-specific expression
dc.subject Heat shock protein gene
dc.subject Transgenic tobacco
dc.subject Nicotiana tabacum
dc.subject Sunflower
dc.subject Zygotic embryogenesis
dc.subject Seed desiccation
dc.subject Mutagenesis
dc.subject Transgene expression
dc.subject Factor
dc.subject Seeds
dc.subject Gene promoters
dc.title An imperfect heat shock element and different upstream sequences are required for the seed-specific expression of a small heat shock protein gene.
dc.type Artículo


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