| dc.creator |
Pelayo, Helvia R. |
|
| dc.creator |
Pincheira, Juana |
|
| dc.creator |
Giménez-Abián, Juan F. |
|
| dc.creator |
Clarke, Duncan J. |
|
| dc.creator |
De la Torre, Consuelo |
|
| dc.date |
2004-09 |
|
| dc.date.accessioned |
2017-01-31T00:58:44Z |
|
| dc.date.available |
2017-01-31T00:58:44Z |
|
| dc.identifier |
Biological Research 2003, vol.36, no.3-4, pp.381-388. |
|
| dc.identifier |
0716-9760 (versión impresa) |
|
| dc.identifier |
10261/2202 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/121788 |
|
| dc.description |
Allium cepa L. meristems were used as a plant model to study the p53-independent control of S and G2 phases by checkpoint pathways, in eukaryotic cells. Checkpoint blocks were induced at early and mid S by hydroxyurea. After their spontaneous override, cells became accumulated in G2-prophase, giving rise later on to a delayed mitotic wave. Cell growth was maintained during the checkpoint blocks, as the delayed mitoses were larger in size than the control ones. Under continuous hydroxyurea treatment, the delayed mitotic was formed by two subpopulations: normal mitoses corresponding to cells having properly recovered from the checkpoint block, and abnormal ones resulting from checkpoint adaptation. These latter cells displayed broken chromatids as they had unduly overriden the G2 checkpoint block, without completing DNA repair. The frequency of the checkpoint-adapted mitoses increased with the hydroxyurea concentration from 0.25 to 1.0 mM. However, from 1 mM hydroxyurea upwards, some of the cells lost their competence for checkpoint adaptation. Therefore, the dose of a genotoxic agent that still allows G2 checkpoint adaptation should always be applied in order to get rid of uncontrolled proliferating cells. This is specially suitable for cells lacking a functional p53 protein. |
|
| dc.description |
The work has been partially supported by the Dirección General de Investigación del Ministerio de Ciencia y Tecnología de España (Project BMC2001-2195), by the Mecesup-Postgrado UCH de Chile (Project 9903). We thank the Agencia Española de Cooperación Internacional del Ministerio de Asuntos Exteriores de España for a fellowship granted to H.R.P. |
|
| dc.description |
Peer reviewed |
|
| dc.language |
eng |
|
| dc.publisher |
Sociedad de Biología de Chile |
|
| dc.rights |
openAccess |
|
| dc.subject |
Checkpoint Adaptation |
|
| dc.subject |
Hydroxyurea |
|
| dc.subject |
S and G2 checkpoints |
|
| dc.subject |
p53-independent checkpoints |
|
| dc.subject |
Allium cepa L. meristems |
|
| dc.title |
p53-Independent checkpoint controls in a plant cell model |
|
| dc.type |
Artículo |
|