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HPLC quantification of biogenic amines in cheeses: correlation with PCR-detection of tyramine-producing microorganisms

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dc.creator Fernández García, María
dc.creator Martínez Linares, Daniel
dc.creator Río Lagar, Beatriz del
dc.creator Ladero Losada, Víctor Manuel
dc.creator Álvarez-González, Miguel Ángel
dc.date 2008-07-09T16:09:28Z
dc.date 2008-07-09T16:09:28Z
dc.date 2007-04-30
dc.date.accessioned 2017-01-31T02:47:37Z
dc.date.available 2017-01-31T02:47:37Z
dc.identifier Journal of Dairy Research 74(3): 276-282 (2007)
dc.identifier 0022-0299
dc.identifier http://hdl.handle.net/10261/5723
dc.identifier 10.1017/S0022029907002488
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5723
dc.description The consumption of food and beverages containing high amounts of biogenic amines (BA) can have toxicological effects. BA found in foods and beverages are synthesized by the microbial decarboxylation of certain amino acids. This paper reports the concentrations of BAs in a number of commercial cheeses, as determined by HPLC. The cheeses studied were made from raw and pasteurized milk of different origin, and were subjected to different ripening periods. BA concentrations were lower in short ripening period than in long ripening period cheeses, and higher in cheeses made from raw milk than in those made from pasteurized milk. The highest BA concentrations were recorded in blue cheeses made from raw milk. Tyramine was the most commonly recorded and abundant BA. The presence of tyramine-producing bacteria was determined by PCR, and a good correlation obtained between the results of this method and tyramine detection by HPLC. These methods could be used to complement one another in the detection and quantification of tyramine in cheese prevention of tyramine accumulation in cheese.
dc.description Peer reviewed
dc.format 22195 bytes
dc.format application/pdf
dc.language eng
dc.publisher Cambridge University Press
dc.rights closedAccess
dc.subject Biogenic amines
dc.subject Tyramine
dc.subject Cheese
dc.subject HPLC
dc.subject PCR
dc.title HPLC quantification of biogenic amines in cheeses: correlation with PCR-detection of tyramine-producing microorganisms
dc.type Artículo


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