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Multiplex PCR for the detection and identification of dairy bacteriophages in milk

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dc.contributor European Commission
dc.contributor Ministerio de Educación y Ciencia (España)
dc.contributor Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología
dc.contributor Consejo Superior de Investigaciones Científicas (España)
dc.creator Río Lagar, Beatriz del
dc.creator Binetti, Ana
dc.creator Martín, M. Cruz
dc.creator Fernández García, María
dc.creator Hernández Magadán, Alfonso
dc.creator Álvarez González, Miguel Ángel
dc.date 2008-07-09T15:29:47Z
dc.date 2008-07-09T15:29:47Z
dc.date 2007-02
dc.date.accessioned 2017-01-31T02:46:49Z
dc.date.available 2017-01-31T02:46:49Z
dc.identifier Food Microbiology 24(1): 75-81 (2007)
dc.identifier 0740-0020
dc.identifier http://hdl.handle.net/10261/5720
dc.identifier 10.1016/j.fm.2006.03.001
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5720
dc.description Bacteriophage infections of starter lactic acid bacteria are a serious risk in the dairy industry. Phage infection can lead to slow lactic acid production or even the total failure of fermentation. The associated economic losses can be substantial. Rapid and sensitive methods are therefore required to detect and identify phages at all stages of the manufacture of fermented dairy products.
dc.description This study describes a simple and rapid multiplex PCR method that, in a single reaction, detects the presence of bacteriophages infecting Streptococcus thermophilus and Lactobacillus delbrueckii, plus three genetically distinct ‘species’ of Lactococcus lactis phages commonly found in dairy plants (P335, 936 and c2). Available bacteriophage genome sequences were examined and the conserved regions used to design five pairs of primers, one for each of the above bacteriophage species. These primers were designed to generate specific fragments of different size depending on the species. Since this method can detect the above phages in untreated milk and can be easily incorporated into dairy industry routines, it might be readily used to earmark contaminated milk for use in processes that do not involve susceptible starter organisms or for use in those that involve phage-deactivating conditions.
dc.description B. del Río, M. C. Martín and M. Fernández are beneficiaries of I3P CSIC contracts financed by the European Social Fund. Alfonso H. Magadán was recipient of a fellowship from the Spanish Ministry of Education and Science. This research was supported by projects PC-CIS01-03 from FICYT, Asturias, Spain (co-financed by CAPSA) and BIO 2002-01458 from the MEC, Spain (co-financed by the FEDER PLAN of the European Union).
dc.description Peer reviewed
dc.format 22195 bytes
dc.format application/pdf
dc.language eng
dc.publisher Elsevier
dc.relation http://dx.doi.org/10.1016/j.fm.2006.03.001
dc.rights closedAccess
dc.subject Dairy bacteriophages
dc.subject Multiplex-PCR
dc.subject Detection
dc.subject Identification
dc.subject Streptococcus thermophilus
dc.subject Lactobacillus delbrueckii
dc.subject Lactococcus lactis
dc.title Multiplex PCR for the detection and identification of dairy bacteriophages in milk
dc.type Artículo

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