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Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese Production

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dc.contributor European Commission
dc.contributor Ministerio de Educación y Ciencia (España)
dc.contributor Consejo Superior de Investigaciones Científicas (España)
dc.creator Fernández García, María
dc.creator Linares, Daniel M.
dc.creator Río Lagar, Beatriz del
dc.creator Martín, M. Cruz
dc.creator Álvarez González, Miguel Ángel
dc.date 2008-07-09T15:10:08Z
dc.date 2008-07-09T15:10:08Z
dc.date 2006
dc.date.accessioned 2017-01-31T02:46:31Z
dc.date.available 2017-01-31T02:46:31Z
dc.identifier J. Dairy Sci. 89(10): 3763-3769 (2006)
dc.identifier 0022-0302
dc.identifier http://hdl.handle.net/10261/5718
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5718
dc.description 7 pages, 1 figure.-- PMID: 16960050 [PubMed].-- Copyright © American Dairy Science Association, 2006.
dc.description Biogenic amines are toxic substances that appear in foods and beverages as a result of AA decarboxylation. The enzyme histidine decarboxylase catalyzes the decarboxylation of histidine to histamine, the biogenic amine most frequently involved in food poisoning. The aim of the present work was to develop a real-time quantitative PCR assay for the direct detection and quantification of histamine-producing strains in milk and cheese. A set of primers was designed, based on the histidine decarboxylase gene sequence of different gram-positive bacteria. The results show the proposed procedure to be a rapid (total processing time < 2 h), specific and highly sensitive technique for detecting potential histamine-producing strains. Chromatographic methods (HPLC) verified the capacity of real-time quantitative PCR to correctly quantify histamine accumulation.
dc.description This work was supported by European Union research grant no. QRLT-2002-02388. D. M. Linares was the recipient of a fellowship from the Spanish Ministry of Education and Science. M. Fernández, B. del Río, and M. C. Martín are beneficiaries of I3P CSIC contracts financed by the European Social Fund.
dc.description Peer reviewed
dc.format 96704 bytes
dc.format application/pdf
dc.language eng
dc.publisher American Dairy Science Association
dc.relation http://jds.fass.org/cgi/content/full/89/10/3763
dc.rights openAccess
dc.subject Histamine detection
dc.subject Lactic Acid Bacteria
dc.subject Cheese
dc.subject Real-Time Quantitative Polymerase Chain Reaction
dc.title Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese Production
dc.type Artículo

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