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Detection and Characterization of Streptococcus thermophilus Bacteriophages by Use of the Antireceptor Gene Sequence

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dc.contributor Ministerio de Educación y Ciencia (España)
dc.contributor European Commission
dc.creator Binetti, Ana
dc.creator Río Lagar, Beatriz del
dc.creator Martín, M. Cruz
dc.creator Álvarez González, Miguel Ángel
dc.date 2008-07-09T07:14:49Z
dc.date 2008-07-09T07:14:49Z
dc.date 2005-10
dc.date.accessioned 2017-01-31T02:43:52Z
dc.date.available 2017-01-31T02:43:52Z
dc.identifier Applied and Environmental Microbiology 71(10): 6096-6103 (2005)
dc.identifier 0099-2240
dc.identifier http://hdl.handle.net/10261/5693
dc.identifier 10.1128/AEM.71.10.6096-6103.2005
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5693
dc.description In the dairy industry, the characterization of Streptococcus thermophilus phage types is very important for the selection and use of efficient starter cultures. The aim of this study was to develop a characterization system useful in phage control programs in dairy plants. A comparative study of phages of different origins was initially performed based on their morphology, DNA restriction profiles, DNA homology, structural proteins, packaging mechanisms, and lifestyles and on the presence of a highly conserved DNA fragment of the replication module. However, these traditional criteria were of limited industrial value, mainly because there appeared to be no correlation between these variables and host ranges. We therefore developed a PCR method to amplify VR2, a variable region of the antireceptor gene, which allowed rapid detection of S. thermophilus phages and classification of these phages. This method has a significant advantage over other grouping criteria since our results suggest that there is a correlation between typing profiles and host ranges. This association could be valuable for the dairy industry by allowing a rational starter rotation system to be established and by helping in the selection of more suitable starter culture resistance mechanisms. The method described here is also a useful tool for phage detection, since specific PCR amplification was possible when phage-contaminated milk was used as a template (detection limit, 105 PFU ml–1).
dc.description This research was supported by project BIO 2002-01458 from MEC, Spain (cofinanced by PLAN FEDER from the European Union).
dc.description Peer reviewed
dc.format 22195 bytes
dc.format application/pdf
dc.language eng
dc.publisher American Society for Microbiology
dc.relation http://dx.doi.org/10.1128/AEM.71.10.6096-6103.2005
dc.rights closedAccess
dc.subject Virus
dc.subject Bacteria
dc.subject Micrococcales
dc.subject Streptococcaceae
dc.subject Phage
dc.subject Nucleotide sequence
dc.subject Streptococcus salivarius subsp. thermophilus
dc.subject Characterization
dc.subject Detection
dc.title Detection and Characterization of Streptococcus thermophilus Bacteriophages by Use of the Antireceptor Gene Sequence
dc.type Artículo

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