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Sequencing, characterization and transcriptional analysis of the histidine decarboxylase operon of Lactobacillus buchneri

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dc.contributor Ministerio de Ciencia y Tecnología (España)
dc.contributor European Commission
dc.creator Martín, M. Cruz
dc.creator Fernández García, María
dc.creator Linares, Daniel M.
dc.creator Álvarez González, Miguel Ángel
dc.date 2008-07-09T07:06:46Z
dc.date 2008-07-09T07:06:46Z
dc.date 2005-04
dc.date.accessioned 2017-01-31T02:43:46Z
dc.date.available 2017-01-31T02:43:46Z
dc.identifier Microbiology 151(4): 1219-1228 (2005)
dc.identifier 1350-0872
dc.identifier http://hdl.handle.net/10261/5691
dc.identifier 10.1099/mic.0.27459-0
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5691
dc.description The GenBank/EMBL/DDBJ accession number for the sequences reported in this paper is AJ749838.-- Final full-text version of the paper available at: http://dx.doi.org/10.1099/mic.0.27459-0.
dc.description The amplification of an internal fragment of the hdcA gene for histidine decarboxylase in Lactobacillus buchneri showed the gene to be located on the bacterial chromosome. Reverse PCR was then used to amplify both it and its adjacent genes. The histidine decarboxylase cluster was found to be composed of four genes: hdcC (expressed in Lactococcus lactis, the product of which is located in the membrane, suggesting it to be a histidine/histamine antiporter), hdcA (which encodes histidine decarboxylase), hdcB (of unknown function but co-transcribed as bicistronic mRNA together with hdcA) and hisS (the only copy of a gene encoding a histidyl-tRNA synthetase in Lb. buchneri). The expression of hisS depends on the histidine concentration of the growth medium, and it can be transcribed as monocistronic or hdcA–hdcB–hisS polycistronic mRNA.
dc.description D. M. Linares was the recipient of a fellowship from the Spanish Ministry of Science and Technology. This research was supported by project QLK1-CT-2002-02388 (European Union).
dc.description Peer reviewed
dc.format 22195 bytes
dc.format application/pdf
dc.language eng
dc.publisher Society for General Microbiology
dc.relation http://dx.doi.org/10.1099/mic.0.27459-0
dc.rights closedAccess
dc.subject BA
dc.subject Biogenic amine
dc.subject LAB
dc.subject Lactic Acid Bacteria
dc.title Sequencing, characterization and transcriptional analysis of the histidine decarboxylase operon of Lactobacillus buchneri
dc.type Artículo

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