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Genetic procedures for identification of enterotoxigenic strains of Staphylococcus aureus from three food poisoning outbreaks

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dc.contributor Fondo de Investigación Sanitaria (España)
dc.creator Martín, M. Cruz
dc.creator Fueyo Mendoza, José María
dc.creator González-Hevia, M. A.
dc.creator Mendoza, M. Carmen
dc.date 2008-07-08T12:47:07Z
dc.date 2008-07-08T12:47:07Z
dc.date 2004-08
dc.date.accessioned 2017-01-31T02:43:03Z
dc.date.available 2017-01-31T02:43:03Z
dc.identifier International Journal of Food Microbiology 94, 3: 279-286 (2004).
dc.identifier http://dx.doi.org/10.1016/j.ijfoodmicro.2004.01.011
dc.identifier 0168-1605
dc.identifier http://hdl.handle.net/10261/5680
dc.identifier 10.1016/j.ijfoodmicro.2004.01.011
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5680
dc.description Final full-text version of the paper available at: http://dx.doi.org/10.1016/j.ijfoodmicro.2004.01.011.
dc.description Three food poisoning restaurant outbreaks due to Staphylococcus aureus, occurring during June–October 2002 in the Principality of Asturias (PA), Spain, provided the basis for investigating some aspects of the molecular epidemiology of this organism. The methods applied to identify strains and lineages included multiplex-polymerase chain reaction (PCR) to detect nine enterotoxin (se) genes, and three DNA fingerprinting procedures: pulsed-field gel electrophoresis (PFGE) with SmaI, randomly amplified polymorphic DNA (RAPD) with two selected primers, and plasmid restriction analysis with HindIII. Thirty-two isolates were differentiated into three non-se and 12 se strains, which were outbreak-specific, except for one that was represented in two of the outbreaks. In outbreak 1, the 16 food isolates analyzed had sec, seg and sei genes and generated a distinctive DNA fingerprint, being assigned to a single strain. This strain could be categorized as endemic in the PA and associated to manually handled dairy products and nasal carriers. In outbreak 2, the four food isolates analyzed fell into three strains, each one displaying a different se-gene profile (sea, sec and seg-seh-sei) and a distinctive DNA fingerprint. In outbreak 3, the five food isolates tested fell into four seg-sei strains generating identical RAPD but different PFGE and plasmid profiles, and one sea strain also collected from two nasal carriers. This last strain had also been found in manually handled vegetables in outbreak 2, and it belongs to a not very frequently found sea lineage in the PA. Multiplex-PCR to detect se genes together with the three applied DNA fingerprint typing procedures proved therefore to be useful tools in subclassifying S. aureus for epidemiological purposes.
dc.description This work has been supported by a grant from the "Fondo de Investigación Sanitaria" PI020172.
dc.description Peer reviewed
dc.format 22195 bytes
dc.format application/pdf
dc.language eng
dc.publisher Elsevier
dc.rights closedAccess
dc.subject Staphylococcus aureus
dc.subject Enterotoxins
dc.subject Food poisoning
dc.subject Outbreaks
dc.subject PCR
dc.subject DNA fingerprinting
dc.title Genetic procedures for identification of enterotoxigenic strains of Staphylococcus aureus from three food poisoning outbreaks
dc.type Artículo


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