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In Situ Molecular Identification of the Plastid ω3 Fatty Acid Desaturase FAD7 from Soybean: Evidence of Thylakoid Membrane Localization

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dc.creator Andreu Gargallo, Vanesa
dc.creator Collados Collados, Raquel
dc.creator Testillano, P. S.
dc.creator Risueño, María Carmen
dc.creator Picorel Castaño, Rafael
dc.creator Alfonso Lozano, Miguel
dc.date 2008-07-03T13:19:15Z
dc.date 2008-07-03T13:19:15Z
dc.date 2007-10
dc.date.accessioned 2017-01-31T02:30:27Z
dc.date.available 2017-01-31T02:30:27Z
dc.identifier Plant Physiology 145:1336-1344(2007)
dc.identifier 0032-0889
dc.identifier http://hdl.handle.net/10261/5575
dc.identifier 10.1104/pp.107.109637
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5575
dc.description ω3 fatty acid desaturases are the enzymes responsible for the synthesis of trienoic fatty acids in plants. These enzymes have been mainly investigated using molecular, biochemical, and genetic approaches but very little is known about their subcellular distribution in plant cells. In this work, the precise subcellular localization of the ω3 desaturase FAD7 was elucidated by immunofluorescence and immunogold labeling using a monospecific GmFAD7 polyclonal antibody in soybean (Glycine max) photoautotrophic cell suspension cultures. Confocal analysis revealed the localization of the GmFAD7 protein within the chloroplast; i.e. signals from FAD7 and chlorophyll autofluorescence showed specific colocalization. Immunogold labeling was pursued on cryofixed and freeze-substituted samples for convenient preservation of antigenicity and ultrastructure of membrane subcompartments. Our data revealed that the FAD7 protein was preferentially localized in the thylakoid membranes. Biochemical fractionation of purified chloroplasts and western analysis of the subfractions further confirmed these results. These findings suggest that not only the envelope, but also the thylakoid membranes could be sites of lipid desaturation in higher plants.
dc.description This work was supported by the Aragón Government (grant no. PIP 140/2005) and the Ministry of Education and Culture of Spain (grant nos. BFU2005–07422–C02–01, AGL2005–05104, BFU2005–01094, and PIP2006–120). R.C. and V.A. were recipients of a predoctoral fellowship from the Aragón Government.
dc.description Peer reviewed
dc.format 22571 bytes
dc.format application/pdf
dc.language eng
dc.publisher American Society of Plant Biologists
dc.rights openAccess
dc.title In Situ Molecular Identification of the Plastid ω3 Fatty Acid Desaturase FAD7 from Soybean: Evidence of Thylakoid Membrane Localization
dc.type Artículo


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