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Cloning and Sequence Analysis of a cDNA Encoding Ferric Leghemoglobin Reductase from Soybean Nodules

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dc.creator Ji, Lin
dc.creator Becana Ausejo, Manuel
dc.creator Sarath, Gautam
dc.creator Klucas, R. V.
dc.date 1994-02
dc.date.accessioned 2017-01-31T02:20:52Z
dc.date.available 2017-01-31T02:20:52Z
dc.identifier PLANT PHYSIOLOGY , Vol 104, Issue 2 453-459
dc.identifier 0032-0889
dc.identifier http://hdl.handle.net/10261/5548
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5548
dc.description Published as Joumal Series No. 10405, Agricultura1 Research Division, University of Nebraska.
dc.description A cDNA encoding soybean (Glycine max [L.] Merr) ferric leghemoglobin reductase (FLbR), an enzyme that is postulated to play and important role in maintaining leghemoglobin in its functional ferrous state, has been cloned and characterized. A group of highly degenerate oligonucleotides deduced from the N-terminal amino acid sequence of FLbR was used to prime the polymerase chain reaction (PCR) on soybean nodule mRNA and cDNA. A full-length clone of FLbR cDNA was isolated by screening a [lambda]gt11 soybean nodule cDNA library using the specific PCR-amplified FLbR cDNA fragment as a probe. The cDNA contained about 1.8 kb and had a coding sequence for 523 amino acids with a predicted molecular mass of 55,729 D, which included a putative 30-residue signal peptide and a 493-residue mature protein. Computer-aided analysis of the deduced FLbR amino acid sequence showed considerable homology (varied from 20-50% with enzymes and species) to dihydrolipoamide dehydrogenase (EC, glutathione reductase (EC, mercuric reductase (EC, and trypanothione reductase (EC in a superfamily of pyridine nucleotide-disulfide oxidoreductases from various organisms. Northern blot analysis using FLbR cDNA as a probe showed that the FLbR gene was expressed in soybean nodules, leaves, roots, and stems, with a greater level of expression in nodules and leaves than in roots and stems. Southern blot analysis of the genomic DNA showed the presence of two homologous FLbR genes in the soybean genome.
dc.description The work was supported by the National Research Initiative Competitive Grants Program/U.S. Department of Agriculture (grant 93-0318) to R.V.K., and by the Direccion General de Investigación, Científica y Técnica CPB92-0058 to M.B.
dc.description Peer reviewed
dc.language eng
dc.publisher American Society of Plant Biologists
dc.rights openAccess
dc.title Cloning and Sequence Analysis of a cDNA Encoding Ferric Leghemoglobin Reductase from Soybean Nodules
dc.type Artículo

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