| dc.creator |
Gogorcena Aoiz, Yolanda |
|
| dc.creator |
Iturbe-Ormaetxe, Iñaki |
|
| dc.creator |
Escuredo, Pedro R. |
|
| dc.creator |
Becana Ausejo, Manuel |
|
| dc.date |
1994-09 |
|
| dc.date.accessioned |
2017-01-31T02:13:50Z |
|
| dc.date.available |
2017-01-31T02:13:50Z |
|
| dc.identifier |
PLANT PHYSIOLOGY , Vol 108, Issue 2 753-759 |
|
| dc.identifier |
0032-0889 |
|
| dc.identifier |
http://hdl.handle.net/10261/5437 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5437 |
|
| dc.description |
The involvement of activated oxygen in the drought-induced damage of pea (Pisum sativum L. cv Frilene) nodules was examined. To this purpose, various pro-oxidant factors, antioxidant enzymes and related metabolites, and markers of oxidative damage were determined in nodules of well-watered (nodule water potential approximately -0.29 MPa) and water-stressed (nodule water potential approximately -2.03 MPa) plants. Water-stressed nodules entered senescence as evidenced by the 30% decrease in leghemoglobin and total soluble protein. Drought also caused a decrease in the activities of catalase (25%), ascorbate peroxidase (18%), dehydroascorbate reductase (15%), glutathione reductase (31%), and superoxide dismutase (30%), and in the contents of ascorbate (59%), reduced (57%) and oxidized (38%) glutathione, NAD+ and NADH (43%), NADP+ (31%), and NADPH (17%). The decline in the antioxidant capacity of nodules may result from a restricted supply of NAD(P)H in vivo for the ascorbate-glutathione pathway and from the Fe-catalyzed Fenton reactions of ascorbate and glutathione with activated oxygen. The 2-fold increase in the content of "catalytic Fe" would also explain the augmented levels of lipid peroxides (2.4-fold) and oxidatively modified proteins (1.4-fold) found in water-stressed nodules because of the known requirement of lipid and protein oxidation for a transition catalytic metal. |
|
| dc.description |
This work was supported by grant PB92-0058 from the Dirección General de Investigación Científica y Técnica of Spain. Y.G., I.I.O., and P.R.E. were the recipients, respectively, of a postdoctoral contract from Ministerio de Educación y Ciencia, a predoctoral fellowship from Gobierno Vasco, and a predoctoral fellowship from Ministerio de Educación y Ciencia. |
|
| dc.description |
Peer reviewed |
|
| dc.language |
eng |
|
| dc.publisher |
American Society of Plant Biologists |
|
| dc.rights |
openAccess |
|
| dc.title |
Antioxidant Defenses against Activated Oxygen in Pea Nodules Subjected to Water Stress |
|
| dc.type |
Artículo |
|