| dc.creator |
Olmeda, David |
|
| dc.creator |
Castel, Susanna |
|
| dc.creator |
Vilaró, Senén |
|
| dc.creator |
Cano, Amparo |
|
| dc.date |
2008-06-26T07:53:53Z |
|
| dc.date |
2008-06-26T07:53:53Z |
|
| dc.date |
2003-07 |
|
| dc.date.accessioned |
2017-01-31T01:54:47Z |
|
| dc.date.available |
2017-01-31T01:54:47Z |
|
| dc.identifier |
Molecular Biology of the Cell 14(7): 2844–2860 (2003) |
|
| dc.identifier |
1059-1524 |
|
| dc.identifier |
http://hdl.handle.net/10261/5356 |
|
| dc.identifier |
10.1091/mbc.E03-01-0865 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5356 |
|
| dc.description |
Copyright © by American Society for Cell Biology.-- Final full-text version of the paper available at: http://www.molbiolcell.org/content/vol14/issue7/ .-- Supplementary material available at: http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=165681&blobname=mbc_14_7_2844__.html |
|
| dc.description |
β-catenin is a multifunctional protein involved in cell-cell adhesion and Wnt signal transduction. β-Catenin signaling has been proposed to act as inducer of cell proliferation in different tumors. However, in some developmental contexts and cell systems β-catenin also acts as a positive modulator of apoptosis. To get additional insights into the role of β-Catenin in the regulation of the cell cycle and apoptosis, we have analyzed the levels and subcellular localization of endogenous β-catenin and its relation with adenomatous polyposis coli (APC) during the cell cycle in S-phase–synchronized epithelial cells. β-Catenin levels increase in S phase, reaching maximum accumulation at late G2/M and then abruptly decreasing as the cells enter into a new G1 phase. In parallel, an increased cytoplasmic and nuclear localization of β-catenin and APC is observed during S and G2 phases. In addition, strong colocalization of APC with centrosomes, but not β-catenin, is detected in M phase. Interestingly, overexpression of a stable form of β-catenin, or inhibition of endogenous β-catenin degradation, in epidermal keratinocyte cells induces a G2 cell cycle arrest and leads to apoptosis. These results support a role for β-catenin in the control of cell cycle and apoptosis at G2/M in normal and transformed epidermal keratinocytes. |
|
| dc.description |
This work was supported by grants from the Comisión Interministerial de Ciencia y Tecnología (SAF98–0085-C03–01 and SAF2001–2819), Instituto de Salud Carlos III (FIS01/1174) and Comunidad Autónoma de Madrid (08.1/0055/2000) to AC. During the realization of this work D.O. was a predoctoral fellow of the Spanish Ministry of Science and Technology. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
1254187 bytes |
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| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
American Society for Cell Biology |
|
| dc.relation |
http://dx.doi.org/10.1091/mbc.E03-01-0865 |
|
| dc.rights |
openAccess |
|
| dc.title |
β-Catenin regulation during the cell cycle: Implications in G2/M and apoptosis |
|
| dc.type |
Artículo |
|