المستودع الأكاديمي جامعة المدينة

Quantitative detection of clostridium tyrobutyricum in milk by real-time PCR

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dc.creator López-Enríquez, Lorena
dc.creator Rodríguez-Lázaro, David
dc.creator Hernández, Marta
dc.date 2008-06-20T07:50:15Z
dc.date 2008-06-20T07:50:15Z
dc.date 2007-06
dc.date.accessioned 2017-01-31T01:44:28Z
dc.date.available 2017-01-31T01:44:28Z
dc.identifier Applied and Environmental Microbiology 73(11): 3747–3751 (2007)
dc.identifier 0099-2240
dc.identifier http://hdl.handle.net/10261/5200
dc.identifier 10.1128/AEM.02642-06
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5200
dc.description We developed a real-time PCR assay for the quantitative detection of Clostridium tyrobutyricum, which has been identified as the major causal agent of late blowing in cheese. The assay was 100% specific, with an analytical sensitivity of 1 genome equivalent in 40% of the reactions. The quantification was linear (R2 > 0.9995) over a 5-log dynamic range, down to 10 genome equivalents, with a PCR efficiency of >0.946. With optimized detergent treatment and enzymatic pretreatment of the sample before centrifugation and nucleic acid extraction, the assay counted down to 300 C. tyrobutyricum spores, with a relative accuracy of 82.98 to 107.68, and detected as few as 25 spores in 25 ml of artificially contaminated raw or ultrahigh-temperature-treated whole milk.
dc.description This work was supported by the Agrarian Experimental Plan of the ITACyL/Junta de Castilla y León and the European Union's Marie-Curie Mobility Program (contract MEIF-CT-2005-0011564). L.L.-E. received a Ph.D. studentship from the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), D.R.-L. is a fellow of the European Union's Marie-Curie Mobility Program, and M.H. holds a contract from the INIA.
dc.description Peer reviewed
dc.format 25095 bytes
dc.format application/pdf
dc.language eng
dc.publisher American Society for Microbiology
dc.relation http://dx.doi.org/10.1128/AEM.02642-06
dc.rights openAccess
dc.title Quantitative detection of clostridium tyrobutyricum in milk by real-time PCR
dc.type Artículo


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