| dc.creator |
López-Enríquez, Lorena |
|
| dc.creator |
Rodríguez-Lázaro, David |
|
| dc.creator |
Hernández, Marta |
|
| dc.date |
2008-06-20T07:50:15Z |
|
| dc.date |
2008-06-20T07:50:15Z |
|
| dc.date |
2007-06 |
|
| dc.date.accessioned |
2017-01-31T01:44:28Z |
|
| dc.date.available |
2017-01-31T01:44:28Z |
|
| dc.identifier |
Applied and Environmental Microbiology 73(11): 3747–3751 (2007) |
|
| dc.identifier |
0099-2240 |
|
| dc.identifier |
http://hdl.handle.net/10261/5200 |
|
| dc.identifier |
10.1128/AEM.02642-06 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5200 |
|
| dc.description |
We developed a real-time PCR assay for the quantitative detection of Clostridium tyrobutyricum, which has been identified as the major causal agent of late blowing in cheese. The assay was 100% specific, with an analytical sensitivity of 1 genome equivalent in 40% of the reactions. The quantification was linear (R2 > 0.9995) over a 5-log dynamic range, down to 10 genome equivalents, with a PCR efficiency of >0.946. With optimized detergent treatment and enzymatic pretreatment of the sample before centrifugation and nucleic acid extraction, the assay counted down to 300 C. tyrobutyricum spores, with a relative accuracy of 82.98 to 107.68, and detected as few as 25 spores in 25 ml of artificially contaminated raw or ultrahigh-temperature-treated whole milk. |
|
| dc.description |
This work was supported by the Agrarian Experimental Plan of the ITACyL/Junta de Castilla y León and the European Union's Marie-Curie Mobility Program (contract MEIF-CT-2005-0011564). L.L.-E. received a Ph.D. studentship from the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), D.R.-L. is a fellow of the European Union's Marie-Curie Mobility Program, and M.H. holds a contract from the INIA. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
25095 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
American Society for Microbiology |
|
| dc.relation |
http://dx.doi.org/10.1128/AEM.02642-06 |
|
| dc.rights |
openAccess |
|
| dc.title |
Quantitative detection of clostridium tyrobutyricum in milk by real-time PCR |
|
| dc.type |
Artículo |
|