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Hob3p, the fission yeast ortholog of human BIN3, localizes Cdc42p to the division site and regulates cytokinesis

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dc.creator Coll, Pedro M.
dc.creator Rincón Padilla, Sergio A.
dc.creator Pérez González, Pilar
dc.creator Izquierdo, Raul
dc.date 2008-06-18T10:50:34Z
dc.date 2008-06-18T10:50:34Z
dc.date 2007-04
dc.date.accessioned 2017-01-31T01:43:43Z
dc.date.available 2017-01-31T01:43:43Z
dc.identifier EMBO J. 2007 April 4; 26(7): 1865–1877
dc.identifier 1460-2075
dc.identifier http://hdl.handle.net/10261/5173
dc.identifier 10.1038/sj.emboj.7601641
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5173
dc.description Final full-text version of the paper available at: http://www.nature.com/emboj/journal/v26/n7/index.html
dc.description Cdc42 GTPase is required for polarization in eukaryotic cells, but its spatial regulation is poorly understood. In Schizosaccharomyces pombe, Cdc42p is activated by Scd1p and Gef1p, two guanine-nucleotide exchange factors. Two-hybrid screening identified Hob3p as a Gef1p binding partner. Hob3p is a BAR domain-containing protein ortholog of human Bin3. Hob3p also interacts directly with Cdc42p independently of Gef1p. Hob3p, Cdc42p and Gef1p form a complex, and Hob3p facilitates Gef1p–Cdc42p interaction and activation. Hob3p forms a ring in the division area, similar to that of Gef1p. This localization requires actin polymerization and Cdc15p but is independent of the septation initiation network. Hob3p is required for the concentration of Cdc42p to the division area. The actomyosin ring contraction is slower in hob3Δ than in wild-type cells, and this contributes to its cytokinesis defect. Moreover, this report extends previous evidence that human Bin3 suppresses the cytokinesis phenotype of hob3Δ cells, showing that Bin3 can partially recover the GTP-Cdc42p level and its localization. These results suggest that Hob3p is required to recruit and activate Cdc42p at the cell division site and that this function might be conserved in other eukaryotes.
dc.description This work was supported by grant BIO2004-0834 from the Comision Interministerial de Ciencia y Tecnología, Spain.
dc.description Peer reviewed
dc.format 22195 bytes
dc.format application/pdf
dc.language eng
dc.publisher Nature Publishing Group
dc.rights openAccess
dc.subject Amphiphysin
dc.subject Cdc42
dc.subject Cytokinesis
dc.subject Fission yeast
dc.subject GEF
dc.subject GTPases
dc.title Hob3p, the fission yeast ortholog of human BIN3, localizes Cdc42p to the division site and regulates cytokinesis
dc.type Artículo

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