| dc.creator |
González-Novo, Alberto |
|
| dc.creator |
Sánchez-Pérez, Miguel |
|
| dc.creator |
Vázquez de Aldana, Carlos R. |
|
| dc.creator |
Jiménez, Javier |
|
| dc.creator |
Correa-Bordes, Jaime |
|
| dc.creator |
Labrador, Leticia |
|
| dc.date |
2008-06-18T10:38:18Z |
|
| dc.date |
2008-06-18T10:38:18Z |
|
| dc.date |
2008-04 |
|
| dc.date.accessioned |
2017-01-31T01:43:26Z |
|
| dc.date.available |
2017-01-31T01:43:26Z |
|
| dc.identifier |
Molecular Biology of the Cell 19(4): 1509–1518 (2008) |
|
| dc.identifier |
1059-1524 |
|
| dc.identifier |
http://hdl.handle.net/10261/5170 |
|
| dc.identifier |
10.1091/mbc.E07-09-0876 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5170 |
|
| dc.description |
Copyright © by American Society for Cell Biology.-- Final full-text version of the paper available at: http://www.molbiolcell.org/content/vol19/issue4/ |
|
| dc.description |
When Candida albicans yeast cells receive the appropriate stimulus, they switch to hyphal growth, characterized by continuous apical elongation and the inhibition of cell separation. The molecular basis of this inhibition is poorly known, despite its crucial importance for hyphal development. In C. albicans, septins are important for hypha formation and virulence. Here, we used fluorescence recovery after photobleaching analysis to characterize the dynamics of septin rings during yeast and hyphal growth. On hyphal induction, septin rings are converted to a hyphal-specific state, characterized by the presence of a frozen core formed by Sep7/Shs1, Cdc3 and Cdc12, whereas Cdc10 is highly dynamic and oscillates between the ring and the cytoplasm. Conversion of septin rings to the hyphal-specific state inhibits the translocation of Cdc14 phosphatase, which controls cell separation, to the hyphal septum. Modification of septin ring dynamics during hyphal growth is dependent on Sep7 and the hyphal-specific cyclin Hgc1, which partially controls Sep7 phosphorylation status and protein levels. Our results reveal a link between the cell cycle machinery and septin cytoskeleton dynamics, which inhibits cell separation in the filaments and is essential for hyphal morphogenesis. |
|
| dc.description |
This work was supported by grants from the Fundación Ramón Areces (to M.S.), Junta de Castilla y Léon (SA013B05) (to J.J.), and from the Ministerio de Ciencia y Tecnología (BFU2006-10318 to J.C.-B. and BFU-2004-00778 to C.R.V.). A.G.-N. held a fellowship from the Ministerio de Ciencia y Tecnología. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
22195 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
American Society for Cell Biology |
|
| dc.relation |
http://dx.doi.org/10.1091/mbc.E07-09-0876 |
|
| dc.rights |
openAccess |
|
| dc.title |
Sep7 Is Essential to Modify Septin Ring Dynamics and Inhibit Cell Separation during Candida albicans Hyphal Growth |
|
| dc.type |
Artículo |
|