| dc.creator |
Cebolla Burillo, Vicente L. |
|
| dc.creator |
Mateos Serrano, Elena |
|
| dc.creator |
Membrado Giner, Luis |
|
| dc.creator |
Vela, Jesús |
|
| dc.creator |
Gálvez Buerba, Eva Mª |
|
| dc.creator |
Matt, Muriel |
|
| dc.creator |
Cossio, Fernando Pedro |
|
| dc.date |
2008-06-18T10:32:34Z |
|
| dc.date |
2008-06-18T10:32:34Z |
|
| dc.date |
2007-02-08 |
|
| dc.date.accessioned |
2017-01-31T01:43:23Z |
|
| dc.date.available |
2017-01-31T01:43:23Z |
|
| dc.identifier |
Journal of Chromatography A, 1146 (2007) 251–257 |
|
| dc.identifier |
0021-9673 |
|
| dc.identifier |
http://hdl.handle.net/10261/5169 |
|
| dc.identifier |
10.1016/j.chroma.2007.01.138 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5169 |
|
| dc.description |
journal page: http://www.elsevier.com/wps/find/journaldescription.cws_home/502688/description#description |
|
| dc.description |
A large number of analytes, including non-fluorescent ones, can be sensitively detected by fluorescence scanning densitometry using silica gel HPTLC plates impregnated with a solution of coralyne cation. This is carried out by the variation, increase or decrease, that the corresponding analyte induces on native coralyne emission at a given excitation wavelength. A similar phenomenon was previously described for berberine cation, and Reichardt's dye probes. However, the sensitivity of coralyne in HPTLC detection of non-fluorescent, structurally different analytes (e.g., long-chain alkanes, alcohols, alkylbromides, neutral lipids) is superior to that of the above-mentioned probes. In this work, the analytical viability of this phenomenon for HPTLC detection using coralyne as a probe is explored, and fluorescent responses of a number of analytes on the coralyne system are rationalized in the light of a previously proposed model. This establishes that the resulting intensity for a probe in the presence of a given compound can be explained as a balance between radiative (contribution of non-specific interactions) and non-radiative processes (specific interactions), the latter producing fluorescence quenching. Experimental results and proposed model suggest that this phenomenon may be general for practically all kinds of analytes |
|
| dc.description |
Spanish Ministerio de Educación
y Ciencia (MEC): project CTQ2005-00227/PPQ
Diputación General de Aragón
(DGA): project PM010 |
|
| dc.description |
Peer reviewed |
|
| dc.format |
478226 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Elsevier |
|
| dc.relation |
http://dx.doi.org/10.1016/j.chroma.2007.01.138 |
|
| dc.rights |
openAccess |
|
| dc.subject |
Detection |
|
| dc.subject |
Fluorescence scanning densitometry |
|
| dc.subject |
Planar chromatography |
|
| dc.title |
Coralyne cation, a fluorescent probe for general detection in planar chromatography |
|
| dc.type |
Artículo |
|