DSpace Repository

Fission Yeast Rho5p GTPase Is a Functional Paralogue of Rho1p That Plays a Role in Survival of Spores and Stationary-Phase Cells

Show simple item record

dc.creator Rincón Padilla, Sergio A.
dc.creator Santos Romero, Beatriz
dc.creator Pérez González, Pilar
dc.date 2008-06-18T09:07:18Z
dc.date 2008-06-18T09:07:18Z
dc.date 2006-03
dc.date.accessioned 2017-01-31T01:42:58Z
dc.date.available 2017-01-31T01:42:58Z
dc.identifier Eukaryot Cell 5(3): 435–446 (2006)
dc.identifier 1535-9778
dc.identifier http://hdl.handle.net/10261/5152
dc.identifier 10.1128/EC.5.3.435-446.2006
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5152
dc.description The Rho GTPase family and their effectors are key regulators involved in many eukaryotic cell functions related to actin organization and polarity establishment. Schizosaccharomyces pombe Rho1p is essential, directly activates the (1,3)-β-d-glucan synthase, and participates in regulation of cell wall growth and morphogenesis. Here we describe the characterization of the fission yeast Rho5p GTPase, highly homologous to Rho1p, sharing 86% identity and 95% similarity. Overexpression of the hyperactive allele rho5-G15V causes a morphological effect similar to that of rho1-G15V, but the penetrance is significantly lower, and overexpression of the dominant-negative allele rho5-T20N causes lysis like that of rho1-T20N. Importantly, overexpression of rho5+ but no other rho genes is able to rescue the lethality of rho1Δ cells. Shutoff experiments indicated that Rho5p can replace Rho1p, but it is not as effective in maintaining cell wall integrity or actin organization. rho5+ expression is hardly detected during log-phase growth but is induced under nutritional starvation conditions. rho5Δ cells are viable and do not display any defects during logarithmic growth. However, when rho1+ expression is repressed during stationary phase, rho5Δ cells display reduced viability. Ascospores lacking Rho5p are less resistant to heat or lytic enzymes than wild-type spores. Moreover, h90 mutant strains carrying the hyperactive rho5-G15V or the dominant-negative rho5-T20N alleles display severe ascospore formation defects. These results suggest that Rho5p functions in a way similar to, but less efficient than, Rho1p, plays a nonessential role during stationary phase, and participates in the spore wall formation.
dc.description S.A.R. was supported by a fellowship from the Spanish Ministerio de Educación. This work was supported by grants BIO-2001-1531 and BIO-2004-00384 from the Comision Interministerial de Ciencia y Tecnología, Spain.
dc.description Peer reviewed
dc.format 25053 bytes
dc.format application/pdf
dc.language eng
dc.publisher American Society for Microbiology
dc.relation http://dx.doi.org/10.1128/EC.5.3.435-446.2006
dc.rights closedAccess
dc.title Fission Yeast Rho5p GTPase Is a Functional Paralogue of Rho1p That Plays a Role in Survival of Spores and Stationary-Phase Cells
dc.type Artículo

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account