| dc.creator |
Agudo-Ibáñez, Lorena |
|
| dc.creator |
Núñez, Fátima |
|
| dc.creator |
Calvo, Fernando |
|
| dc.creator |
Berenjeno, Inmaculada M. |
|
| dc.creator |
Bustelo, Xosé R. |
|
| dc.creator |
Crespo, Piero |
|
| dc.date |
2008-06-17T06:52:42Z |
|
| dc.date |
2008-06-17T06:52:42Z |
|
| dc.date |
2007-11 |
|
| dc.date.accessioned |
2017-01-31T01:42:11Z |
|
| dc.date.available |
2017-01-31T01:42:11Z |
|
| dc.identifier |
Cellular Signalling 19(11): 2264–2276 (2007) |
|
| dc.identifier |
0898-6568 |
|
| dc.identifier |
http://hdl.handle.net/10261/5124 |
|
| dc.identifier |
10.1016/j.cellsig.2007.06.025 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5124 |
|
| dc.description |
El pdf del artículo es el manuscrito de autor. |
|
| dc.description |
Ras proteins are distributed in distinct plasma-membrane microdomains and endomembranes. The biochemical signals generated by Ras therein differ qualitatively and quantitatively, but the extent to which this spatial variability impacts on the genetic program switched-on by Ras is unknown. We have used microarray technology to identify the transcriptional targets of localization-specific Ras subsignals in NIH3T3 cells expressing H-RasV12 selectively tethered to distinct cellular microenvironments. We report that the transcriptomes resulting from site-specific Ras activation show a significant overlap. However, distinct genetic signatures can also be found for each of the Ras subsignals. Our analyses unveil 121 genes uniquely regulated by Ras signals emanating from plasma-membrane microdomains. Interestingly, not a single gene is specifically controlled by lipid raft-anchored Ras. Furthermore, only 9 genes are exclusive for Ras signals from endomembranes. Also, we have identified 31 genes common to the site-specific Ras subsignals capable of inducing cellular transformation. Among these are the genes coding for Vitamin D receptor and for p120-GAP and we have assessed their impact in Ras-induced transformation. Overall, this report reveals the complexity and variability of the different genetic programs orchestrated by Ras from its main sublocalizations. |
|
| dc.description |
PC’s work is supported by grants from the Spanish Ministry
of Education and Science (MES) (BFU2005-00777 and GEN2003-20239-C06-03), the EU Sixth Framework Program
under the SIMAP project, and the Red Temática de Investigación Cooperativa en Cáncer (RTICC) (RD06/0020/0105).
Fondo de Investigaciones Sanitarias (FIS), Carlos III Institute, Spanish Ministry of Health. XRB’s work is supported
by grants from the US National Cancer Institute/NIH (5R01-CA73735-10), the MES (SAF2006-01789 and
GEN2003-20239-C06-01), the Castilla-León Autonomous Government (SA053A05), and the RTICC
(RD06/0020/0001). F.N. was partially supported by a fellowship by the Ernst Schering Foundation. LA, FC, and IMB
are Spanish Ministry of Education predoctoral fellows. All Spanish funding is co-sponsored by the European Union. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
2193886 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Elsevier |
|
| dc.relation |
http://dx.doi.org/10.1016/j.cellsig.2007.06.025 |
|
| dc.rights |
openAccess |
|
| dc.subject |
Ras |
|
| dc.subject |
Compartmentalization |
|
| dc.subject |
Gene microarrays |
|
| dc.subject |
Transformation |
|
| dc.title |
Transcriptomal profiling of site-specific Ras signals |
|
| dc.type |
Artículo |
|