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Resistance of virus to extinction on bottleneck passages: Study of a decaying and fluctuating pattern of fitness loss

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dc.contributor European Commission
dc.contributor Fundación Ramón Areces
dc.contributor Ministerio de Ciencia y Tecnología (España)
dc.contributor Comunidad de Madrid
dc.contributor Instituto Nacional de Técnica Aeroespacial (España)
dc.creator Lázaro, Ester
dc.creator Escarmís, Cristina
dc.creator Pérez-Mercader, Juan
dc.creator Manrubia Cuevas, Susanna
dc.creator Domingo, Esteban
dc.date 2008-06-16T06:32:12Z
dc.date 2008-06-16T06:32:12Z
dc.date 2003-09
dc.date.accessioned 2017-01-31T01:41:31Z
dc.date.available 2017-01-31T01:41:31Z
dc.identifier Proceedings of the National Academy of Sciences 100(19): 10830–10835 (2003)
dc.identifier 1091-6490
dc.identifier http://hdl.handle.net/10261/5099
dc.identifier 10.1073/pnas.1332668100
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5099
dc.description Copyright © by National Academy of Sciences. La versión original está disponible en http://www.pnas.org/
dc.description RNA viruses display high mutation rates and their populations replicate as dynamic and complex mutant distributions, termed viral quasispecies. Repeated genetic bottlenecks, which experimentally are carried out through serial plaque-to-plaque transfers of the virus, lead to fitness decrease (measured here as diminished capacity to produce infectious progeny). Here we report an analysis of fitness evolution of several low fitness foot-and-mouth disease virus clones subjected to 50 plaque-to-plaque transfers. Unexpectedly, fitness decrease, rather than being continuous and monotonic, displayed a fluctuating pattern, which was influenced by both the virus and the state of the host cell as shown by effects of recent cell passage history. The amplitude of the fluctuations increased as fitness decreased, resulting in a remarkable resistance of virus to extinction. Whereas the frequency distribution of fitness in control (independent) experiments follows a log-normal distribution, the probability of fitness values in the evolving bottlenecked populations fitted a Weibull distribution. We suggest that multiple functions of viral genomic RNA and its encoded proteins, subjected to high mutational pressure, interact with cellular components to produce this nontrivial, fluctuating pattern.
dc.description Work at the Centro de Biología Molecular Severo Ochoa was supported by BMC Grant 2001-1823-C02-01, Unión Europea Grant PSS 0884, and the Fundación Ramón Areces. S.C.M. acknowledges the Ministerio de Ciencia y Tecnología for a Ramón y Cajal Contract. Work at the Centro de Astrobiolgía was supported by the European Union, the Ministerio de Ciencia y Technología, the Comunidad Autonoma de Madrid, and the Instituto Nacional de Técnica Aeroespacial.
dc.description Peer reviewed
dc.format 22195 bytes
dc.format application/pdf
dc.language eng
dc.publisher National Academy of Sciences (U.S.)
dc.rights closedAccess
dc.title Resistance of virus to extinction on bottleneck passages: Study of a decaying and fluctuating pattern of fitness loss
dc.type Artículo

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