| dc.contributor |
Ministerio de Economía y Competitividad (España) |
|
| dc.contributor |
Fundación Ramón Areces |
|
| dc.creator |
Molina, Susana |
|
| dc.creator |
Sanz, Miguel Ángel |
|
| dc.creator |
Madan, Vanesa |
|
| dc.creator |
Ventoso, Iván |
|
| dc.creator |
Castelló, Alfredo |
|
| dc.creator |
Carrasco Llamas, Luis |
|
| dc.date |
2008-06-12T14:30:07Z |
|
| dc.date |
2008-06-12T14:30:07Z |
|
| dc.date |
2007-03-23 |
|
| dc.date.accessioned |
2017-01-31T01:40:50Z |
|
| dc.date.available |
2017-01-31T01:40:50Z |
|
| dc.identifier |
Virology, Vol. 363, Issue 2, 5 July 2007, Pages 430-436 |
|
| dc.identifier |
0042-6822 (Print) |
|
| dc.identifier |
1096-0341 (Online) |
|
| dc.identifier |
http://hdl.handle.net/10261/5048 |
|
| dc.identifier |
10.1016/j.virol.2007.02.001 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5048 |
|
| dc.description |
Brefeldin A is a macrolide compound that interferes with the secretory pathway and also affects protein synthesis in mammalian cells. As a result, this antibiotic impedes the maturation of viral glycoproteins of enveloped viruses and viral genome replication in several virus species. In the present work, we show that translation of subgenomic mRNA from Sindbis virus, which in contrast to cellular translation is resistant to brefeldin A after prolonged treatment. The phosphorylation of eIF2 as a result of brefeldin A treatment correlates with the inhibition of cellular translation, while late viral protein synthesis is resistant to this phosphorylation. The effect of brefeldin A on Sindbis virus replication was also examined using a Sindbis virus replicon. Although brefeldin A delayed viral RNA synthesis, translation by non-replicative viral RNAs was not affected, reinforcing the idea that brefeldin A delays viral RNA replication, but does not directly affect Sindbis virus protein synthesis |
|
| dc.description |
This study was supported by Grant (BFU2006-02182/BMC) from DGICYT and an Institutional Grant awarded to the Centro de Biología Molecular “Severo Ochoa” by the Fundación Ramón Areces |
|
| dc.description |
Peer reviewed |
|
| dc.format |
179958 bytes |
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| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Elsevier |
|
| dc.relation |
http://dx.doi.org/10.1016/j.virol.2007.02.001 |
|
| dc.rights |
openAccess |
|
| dc.subject |
Brefeldin A |
|
| dc.subject |
Sindbis virus |
|
| dc.subject |
Cellular translation |
|
| dc.subject |
Viral protein synthesis |
|
| dc.subject |
Sindbis virus replication |
|
| dc.title |
Differential inhibition of cellular and Sindbis virus translation by brefeldin A |
|
| dc.type |
Artículo |
|