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Differential inhibition of cellular and Sindbis virus translation by brefeldin A

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dc.contributor Ministerio de Economía y Competitividad (España)
dc.contributor Fundación Ramón Areces
dc.creator Molina, Susana
dc.creator Sanz, Miguel Ángel
dc.creator Madan, Vanesa
dc.creator Ventoso, Iván
dc.creator Castelló, Alfredo
dc.creator Carrasco Llamas, Luis
dc.date 2008-06-12T14:30:07Z
dc.date 2008-06-12T14:30:07Z
dc.date 2007-03-23
dc.date.accessioned 2017-01-31T01:40:50Z
dc.date.available 2017-01-31T01:40:50Z
dc.identifier Virology, Vol. 363, Issue 2, 5 July 2007, Pages 430-436
dc.identifier 0042-6822 (Print)
dc.identifier 1096-0341 (Online)
dc.identifier http://hdl.handle.net/10261/5048
dc.identifier 10.1016/j.virol.2007.02.001
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5048
dc.description Brefeldin A is a macrolide compound that interferes with the secretory pathway and also affects protein synthesis in mammalian cells. As a result, this antibiotic impedes the maturation of viral glycoproteins of enveloped viruses and viral genome replication in several virus species. In the present work, we show that translation of subgenomic mRNA from Sindbis virus, which in contrast to cellular translation is resistant to brefeldin A after prolonged treatment. The phosphorylation of eIF2 as a result of brefeldin A treatment correlates with the inhibition of cellular translation, while late viral protein synthesis is resistant to this phosphorylation. The effect of brefeldin A on Sindbis virus replication was also examined using a Sindbis virus replicon. Although brefeldin A delayed viral RNA synthesis, translation by non-replicative viral RNAs was not affected, reinforcing the idea that brefeldin A delays viral RNA replication, but does not directly affect Sindbis virus protein synthesis
dc.description This study was supported by Grant (BFU2006-02182/BMC) from DGICYT and an Institutional Grant awarded to the Centro de Biología Molecular “Severo Ochoa” by the Fundación Ramón Areces
dc.description Peer reviewed
dc.format 179958 bytes
dc.format application/pdf
dc.language eng
dc.publisher Elsevier
dc.relation http://dx.doi.org/10.1016/j.virol.2007.02.001
dc.rights openAccess
dc.subject Brefeldin A
dc.subject Sindbis virus
dc.subject Cellular translation
dc.subject Viral protein synthesis
dc.subject Sindbis virus replication
dc.title Differential inhibition of cellular and Sindbis virus translation by brefeldin A
dc.type Artículo

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