DSpace Repository

The ectopic overexpression of a seed-specific transcription factor, HaHSFA9, confers tolerance to severe dehydration in vegetative organs.

Show simple item record

dc.creator Prieto-Dapena, P.
dc.creator Castaño, Raúl
dc.creator Almoguera, Concepción
dc.creator Jordano, Juan
dc.date 2008-06-12T06:33:11Z
dc.date 2008-06-12T06:33:11Z
dc.date 2008-06
dc.date.accessioned 2017-01-31T01:39:28Z
dc.date.available 2017-01-31T01:39:28Z
dc.identifier The Plant Journal 54 (6) , 1004–1014
dc.identifier 1004–1014
dc.identifier http://hdl.handle.net/10261/5005
dc.identifier 10.1111/j.1365-313X.2008.03465.x
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5005
dc.description This article has been evaluated in Faculty of 1000 Biology. ISSN 1740-4118
dc.description Most plant seeds tolerate desiccation, but vegetative tissues are intolerant to drastic dehydration, except in the case of resurrection plants. Therefore, changes in the regulation of genes normally expressed in seeds are thought to be responsible for the evolutionary origin of desiccation tolerance in resurrection plants. Here, we show that constitutive overexpression of the seed-specific HSFA9 transcription factor from sunflower is sufficient to confer tolerance to severe dehydration, outside of the developing seed context, to vegetative tissues of transgenic tobacco. Whole 3-week-old seedlings could survive severe dehydration. This was quantified as a water loss to 1.96 ± 0.05% of the initial water content, which corresponds to a water potential of ≈ −40 MPa. Survival depended on the water potential, from 40% survival at ≈ −20 MPa to 6.5% survival at ≈ −40 MPa. Whole-seedling survival was limited by the dehydration sensitivity of the roots. Survival correlated with the ectopic expression of a genetic program involving seed-specific, small heat-shock proteins, but not late embryogenesis abundant proteins. The accumulation of sucrose or raffinose family oligosaccharides was not altered by HSFA9. The observed tolerance was achieved without a reduction of growth and development. Our results strongly support the previously suggested contribution of small heat-shock proteins to the desiccation tolerance of seeds. We provide a successful system for analyzing tolerance to severe dehydration in all vegetative organs of seedlings. We propose that HSFA9 is a potential genetic switch involved in the evolution of tolerance to vegetative desiccation.
dc.description Peer reviewed
dc.format 264985 bytes
dc.format 219953 bytes
dc.format 788876 bytes
dc.format 863650 bytes
dc.format 468788 bytes
dc.format 46498 bytes
dc.format 70263 bytes
dc.format 16148 bytes
dc.format application/pdf
dc.format application/pdf
dc.format application/pdf
dc.format application/pdf
dc.format application/pdf
dc.format application/pdf
dc.format application/pdf
dc.format application/pdf
dc.language eng
dc.publisher Blackwell Publishing
dc.relation http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2008.03465.x/pdf
dc.rights closedAccess
dc.subject Desiccation
dc.subject Vegetative tolerance
dc.subject Seeds
dc.subject HSFA9
dc.subject Genetic switch
dc.title The ectopic overexpression of a seed-specific transcription factor, HaHSFA9, confers tolerance to severe dehydration in vegetative organs.
dc.type Artículo


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account