| dc.contributor |
Ministerio de Ciencia y Tecnología (España) |
|
| dc.contributor |
Comunidad de Madrid |
|
| dc.contributor |
Genetrix |
|
| dc.contributor |
Consejo Superior de Investigaciones Científicas (España) |
|
| dc.contributor |
Fundación Ramón Areces |
|
| dc.contributor |
European Commission |
|
| dc.contributor |
Instituto Nacional de Técnica Aeroespacial (España) |
|
| dc.contributor |
Ministerio de Educación y Ciencia (España) |
|
| dc.creator |
Martín, Verónica |
|
| dc.creator |
Perales, Celia |
|
| dc.creator |
Abia, David |
|
| dc.creator |
Ortiz, Ángel R. |
|
| dc.creator |
Domingo, Esteban |
|
| dc.creator |
Briones, Carlos |
|
| dc.date |
2008-06-10T13:49:30Z |
|
| dc.date |
2008-06-10T13:49:30Z |
|
| dc.date |
2006-05-18 |
|
| dc.date.accessioned |
2017-01-31T01:38:38Z |
|
| dc.date.available |
2017-01-31T01:38:38Z |
|
| dc.identifier |
BMC Genomics 2006, 7:117 |
|
| dc.identifier |
1471-2164 |
|
| dc.identifier |
http://hdl.handle.net/10261/4958 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4958 |
|
| dc.description |
Article available at http://dx.doi.org/10.1186/1471-2164-7-117 |
|
| dc.description |
[Background] The evolution of viral quasispecies can influence viral pathogenesis and the response
to antiviral treatments. Mutant clouds in infected organisms represent the first stage in the genetic
and antigenic diversification of RNA viruses, such as foot and mouth disease virus (FMDV), an
important animal pathogen. Antigenic variants of FMDV have been classically diagnosed by
immunological or RT-PCR-based methods. DNA microarrays are becoming increasingly useful for
the analysis of gene expression and single nucleotide polymorphisms (SNPs). Recently, a FMDV
microarray was described to detect simultaneously the seven FMDV serotypes. These results
encourage the development of new oligonucleotide microarrays to probe the fine genetic and
antigenic composition of FMDV for diagnosis, vaccine design, and to gain insight into the molecular
epidemiology of this pathogen. |
|
| dc.description |
[Results] A FMDV microarray was designed and optimized to detect SNPs at a major antigenic site
of the virus. A screening of point mutants of the genomic region encoding antigenic site A of FMDV
C-S8c1 was achieved. The hybridization pattern of a mutant includes specific positive and negative
signals as well as crosshybridization signals, which are of different intensity depending on the
thermodynamic stability of each probe-target pair. Moreover, an array bioinformatic classification
method was developed to evaluate the hybridization signals. This statistical analysis shows that the
procedure allows a very accurate classification per variant genome. |
|
| dc.description |
[Conclusion] A specific approach based on a microarray platform aimed at distinguishing point
mutants within an important determinant of antigenicity and host cell tropism, namely the G-H loop
of capsid protein VP1, was developed. The procedure is of general applicability as a test for
specificity and discriminatory power of microarray-based diagnostic procedures using multiple
oligonucleotide probes. |
|
| dc.description |
Work supported by grants BMC 2001-1823-C02-01, CAM 08.2/0015/
2001.1, PROFIT 2003 awarded to Genetrix S.L. (FIT 010000-2002-38),
FIS2004-06414, BFU 2005-00863, GEN2001-4865-C13-10, GEN2001-
4856-C13-07, a CSIC contract I3P-PC2004L and an institutional grant from
Fundación Ramón Areces. Work at Centro de Astrobiología was also supported
by EU, INTA, MEC and CAM. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
570044 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
BioMed Central |
|
| dc.relation |
Publisher’s version |
|
| dc.rights |
openAccess |
|
| dc.subject |
Foot-and-mouth disease virus |
|
| dc.subject |
Microarray |
|
| dc.title |
Microarray-based identification of antigenic variants of foot-and-mouth disease virus: a bioinformatics quality assessment |
|
| dc.type |
Artículo |
|