المستودع الأكاديمي جامعة المدينة

Hedgehog lipid modifications are required for Hedgehog stabilization in the extracellular matrix

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dc.contributor Ministerio de Educación y Ciencia (España)
dc.contributor Comunidad de Madrid
dc.contributor Fundación Ramón Areces
dc.creator Callejo de Prado, Ainhoa Itziar
dc.creator Torroja, Carlos
dc.creator Quijada, Luis
dc.creator Guerrero Vega, Isabel
dc.date 2008-06-04T14:11:29Z
dc.date 2008-06-04T14:11:29Z
dc.date 2006-01-05
dc.date.accessioned 2017-01-31T01:34:06Z
dc.date.available 2017-01-31T01:34:06Z
dc.identifier Development 133, 471-483 (2006)
dc.identifier 0950-1991 (Print)
dc.identifier 1477-9129 (Online)
dc.identifier http://hdl.handle.net/10261/4799
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4799
dc.description Article available http://dx.dpi.org/10.1242/dev.02217
dc.description The Hedgehog (Hh) family of morphogenetic proteins has important instructional roles in metazoan development. Despite Hh being modified by Ct-cholesterol and Nt-palmitate adducts, Hh migrates far from its site of synthesis and programs cellular outcomes, depending on its local concentrations. We show that in the receiving cells of the Drosophila wing imaginal disc, lipid-unmodified Hh spreads across many more cell diameters than the wild type and this spreading leads to the activation of low but not high threshold responses. Unlipidated Hh forms become internalized through the apical plasma membrane, while wild-type Hh enters through the basolateral cell surface - in all cases via a dynamin-dependent mechanism. Full activation of the Hh pathway and the spread of Hh throughout the extracellular matrix depend on the ability of lipid-modified Hh to interact with heparan sulfate proteoglycans (HSPG). However, neither Hh-lipid modifications nor HSPG function are required to activate the targets that respond to low levels of Hh. All these data show that the interaction of lipid-modified Hh with HSPG is important both for precise Hh spreading through the epithelium surface and for correct Hh reception
dc.description This study was financed by the Spanish D.G.I.C.Y.T, grants BFU2005-04183/BMC and MCYT GEN2001-4846-C05-01, the Comunidad Autónoma de Madrid, grant GR/SAL/0185/2004, and by an institutional grant from the Fundación Areces.
dc.description Peer reviewed
dc.format 4520273 bytes
dc.format application/pdf
dc.language eng
dc.publisher Company of Biologists
dc.rights openAccess
dc.subject Hedgehog gradient
dc.subject Drosophila imaginal discs
dc.subject Hedgehog lipids
dc.subject HSPG
dc.subject Extracellular matrix
dc.title Hedgehog lipid modifications are required for Hedgehog stabilization in the extracellular matrix
dc.type Artículo


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