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Unraveling the evolutionary history of the phosphoryl-transfer chain of the phosphoenolpyruvate:phosphotransferase system through phylogenetic analyses and genome context

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dc.creator Zúñiga, Manuel
dc.creator Comas, Iñaki
dc.creator González Candelas, Fernando
dc.date 2008-06-03T12:51:24Z
dc.date 2008-06-03T12:51:24Z
dc.date 2008-05-16
dc.date.accessioned 2017-01-31T01:33:30Z
dc.date.available 2017-01-31T01:33:30Z
dc.identifier BMC Evolutionary Biology 2008, 8:147
dc.identifier 1471-2148
dc.identifier http://hdl.handle.net/10261/4778
dc.identifier 10.1186/1471-2148-8-147
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4778
dc.description [Background] The phosphoenolpyruvate phosphotransferase system (PTS) plays a major role in sugar transport and in the regulation of essential physiological processes in many bacteria. The PTS couples solute transport to its phosphorylation at the expense of phosphoenolpyruvate (PEP) and it consists of general cytoplasmic phosphoryl transfer proteins and specific enzyme II complexes which catalyze the uptake and phosphorylation of solutes. Previous studies have suggested that the evolution of the constituents of the enzyme II complexes has been driven largely by horizontal gene transfer whereas vertical inheritance has been prevalent in the general phosphoryl transfer proteins in some bacterial groups. The aim of this work is to test this hypothesis by studying the evolution of the phosphoryl transfer proteins of the PTS.
dc.description [Results] We have analyzed the evolutionary history of the PTS phosphoryl transfer chain (PTS-ptc) components in 222 complete genomes by combining phylogenetic methods and analysis of genomic context. Phylogenetic analyses alone were not conclusive for the deepest nodes but when complemented with analyses of genomic context and functional information, the main evolutionary trends of this system could be depicted.
dc.description [Conclusion] The PTS-ptc evolved in bacteria after the divergence of early lineages such as Aquificales, Thermotogales and Thermus/Deinococcus. The subsequent evolutionary history of the PTS-ptc varied in different bacterial lineages: vertical inheritance and lineage-specific gene losses mainly explain the current situation in Actinobacteria and Firmicutes whereas horizontal gene transfer (HGT) also played a major role in Proteobacteria. Most remarkably, we have identified a HGT event from Firmicutes or Fusobacteria to the last common ancestor of the Enterobacteriaceae, Pasteurellaceae, Shewanellaceae and Vibrionaceae. This transfer led to extensive changes in the metabolic and regulatory networks of these bacteria including the development of a novel carbon catabolite repression system. Hence, this example illustrates that HGT can drive major physiological modifications in bacteria.
dc.description This work was partially funded by a C.S.I.C. project (Ref. 2006 7 0I 097), project BFU2005-00503 from Ministerio de Educación y Ciencia (Spain) and project Grupos 03/2004 from Generalitat Valencia (Spain).
dc.description Peer reviewed
dc.format 1141169 bytes
dc.format application/pdf
dc.language eng
dc.publisher BioMed Central
dc.relation Publisher’s version
dc.rights openAccess
dc.title Unraveling the evolutionary history of the phosphoryl-transfer chain of the phosphoenolpyruvate:phosphotransferase system through phylogenetic analyses and genome context
dc.type Artículo

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