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Regulation of decapentaplegic expression during Drosophila wing veins pupal development

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dc.contributor EMBO
dc.contributor Ministerio de Economía y Competitividad (España)
dc.contributor Fundación Ramón Areces
dc.creator Sotillos, Sol
dc.creator Celis, José F. de
dc.date 2008-06-02T14:38:10Z
dc.date 2008-06-02T14:38:10Z
dc.date 2006-01-18
dc.date.accessioned 2017-01-31T01:32:29Z
dc.date.available 2017-01-31T01:32:29Z
dc.identifier Mechanisms of Development Vol.123, Issue 3, March 2006, Pages 241-251
dc.identifier 0925-4773 (Print)
dc.identifier 1872-6356 (Online)
dc.identifier http://hdl.handle.net/10261/4735
dc.identifier 10.1016/j.mod.2005.12.002
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4735
dc.description The differentiation of veins in the Drosophila wing relies on localised expression of decapentaplegic (dpp) in pro-vein territories during pupal development. The expression of dpp in the pupal veins requires the integrity of the shortvein region (shv), localised 5′ to the coding region. It is likely that this DNA integrates positive and negative regulatory signals directing dpp transcription during pupal development. Here, we identify a minimal 0.9 kb fragment giving localised expression in the vein L5 and a 0.5 kb fragment giving expression in all longitudinal veins. Using a combination of in vivo expression of reporter genes regulated by shv sequences, in vitro binding assays and sequence comparisons between the shv region of different Drosophila species, we found binding sites for the vein-specific transciption factors Araucan, Knirps and Ventral veinless, as well as binding sites for the Dpp pathway effectors Mad and Med. We conclude that conserved vein-specific enhancers regulated by transcription factors expressed in individual veins collaborate with general vein and intervein regulators to establish and maintain the expression of dpp confined to the veins during pupal development
dc.description A postdoctoral short-term fellowship from the European Molecular Biology Organization (EMBO) to S. S. is acknowledged. Grants from Dirección General de Investigación Científica y Técnica to James Castelli-Gair (BFU2004-01069/BMC), to J.F.d C. (BCM2003-1191 and GEN2001-4846-C05-01), as well as an institutional grant from Fundación Ramón Areces to the Centro de Biología Molecular “Severo Ochoa” are also acknowledged
dc.description Peer reviewed
dc.format 696706 bytes
dc.format application/pdf
dc.language eng
dc.publisher Elsevier
dc.relation http://dx.doi.org/10.1016/j.mod.2005.12.002
dc.rights openAccess
dc.subject Drosophila
dc.subject Decapentaplegic
dc.subject Vein differentiation
dc.subject Gene regulation
dc.subject Wing
dc.title Regulation of decapentaplegic expression during Drosophila wing veins pupal development
dc.type Artículo

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