dc.contributor |
Ministerio de Educación y Ciencia (España) |
|
dc.contributor |
Comunidad de Madrid |
|
dc.contributor |
Fundación Ramón Areces |
|
dc.creator |
Navas, Luis de |
|
dc.creator |
Foronda, David |
|
dc.creator |
Suzanne, Magali |
|
dc.creator |
Sánchez-Herrero, Ernesto |
|
dc.date |
2008-05-30T11:58:45Z |
|
dc.date |
2008-05-30T11:58:45Z |
|
dc.date |
2006-08-02 |
|
dc.date.accessioned |
2017-01-31T01:31:15Z |
|
dc.date.available |
2017-01-31T01:31:15Z |
|
dc.identifier |
Mechanisms of Development, Volume 123, Issue 11, November 2006, Pages 860-867 |
|
dc.identifier |
1872-6356 (Online) |
|
dc.identifier |
0925-4773 (Print) |
|
dc.identifier |
http://hdl.handle.net/10261/4693 |
|
dc.identifier |
10.1016/j.mod.2006.07.010 |
|
dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4693 |
|
dc.description |
The functional replacement of one gene product by another one is a powerful method to study specificity in development and evolution. In Drosophila, the Gal4/UAS method has been used to analyze in vivo such functional substitutions. To this aim, Gal4 lines that inactivate a gene and reproduce its expression pattern are required, and they can be frequently obtained by replacing pre-existing P-lacZ lines with such characteristics. We have devised a new method to quickly identify replacements of P-lacZ lines by P-Gal4 lines, and applied it successfully to obtain Gal4 insertions in the Ultrabithorax and Abdominal-B Hox genes. We have used these lines to study the functional replacement of a Hox gene by another one. Our experiments confirm that the abdominal-A gene can replace Ultrabithorax in haltere development but that it cannot substitute for Abdominal-B in the formation of the genitalia. |
|
dc.description |
This work was supported by grants from the Ministerio de Educación y Ciencia (BMC2002-00300), the Comunidad Autónoma de Madrid (No. 08.1/0031/2001.1 and GR/SAL/0147/2004) and an Institutional Grant from the Fundación Ramón Areces. |
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dc.description |
Peer reviewed |
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dc.format |
1868034 bytes |
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dc.format |
958179 bytes |
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dc.format |
148169 bytes |
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dc.format |
283789 bytes |
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dc.format |
1644438 bytes |
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dc.format |
408652 bytes |
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dc.format |
application/pdf |
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dc.format |
application/pdf |
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dc.format |
application/pdf |
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dc.format |
application/pdf |
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dc.format |
application/pdf |
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dc.format |
application/pdf |
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dc.language |
eng |
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dc.publisher |
Elsevier |
|
dc.relation |
http://dx.doi.org/10.1016/j.mod.2006.07.010 |
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dc.rights |
openAccess |
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dc.subject |
Drosophila |
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dc.subject |
P-element |
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dc.subject |
Bithorax complex |
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dc.subject |
Gal4 lines |
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dc.subject |
Ubx |
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dc.subject |
Abd-B |
|
dc.title |
A simple and efficient method to identify replacements of P-lacZ by P-Gal4 lines allows obtaining Gal4 insertions in the bithorax complex of Drosophila |
|
dc.type |
Artículo |
|