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A simple and efficient method to identify replacements of P-lacZ by P-Gal4 lines allows obtaining Gal4 insertions in the bithorax complex of Drosophila

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dc.contributor Ministerio de Educación y Ciencia (España)
dc.contributor Comunidad de Madrid
dc.contributor Fundación Ramón Areces
dc.creator Navas, Luis de
dc.creator Foronda, David
dc.creator Suzanne, Magali
dc.creator Sánchez-Herrero, Ernesto
dc.date 2008-05-30T11:58:45Z
dc.date 2008-05-30T11:58:45Z
dc.date 2006-08-02
dc.date.accessioned 2017-01-31T01:31:15Z
dc.date.available 2017-01-31T01:31:15Z
dc.identifier Mechanisms of Development, Volume 123, Issue 11, November 2006, Pages 860-867
dc.identifier 1872-6356 (Online)
dc.identifier 0925-4773 (Print)
dc.identifier http://hdl.handle.net/10261/4693
dc.identifier 10.1016/j.mod.2006.07.010
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4693
dc.description The functional replacement of one gene product by another one is a powerful method to study specificity in development and evolution. In Drosophila, the Gal4/UAS method has been used to analyze in vivo such functional substitutions. To this aim, Gal4 lines that inactivate a gene and reproduce its expression pattern are required, and they can be frequently obtained by replacing pre-existing P-lacZ lines with such characteristics. We have devised a new method to quickly identify replacements of P-lacZ lines by P-Gal4 lines, and applied it successfully to obtain Gal4 insertions in the Ultrabithorax and Abdominal-B Hox genes. We have used these lines to study the functional replacement of a Hox gene by another one. Our experiments confirm that the abdominal-A gene can replace Ultrabithorax in haltere development but that it cannot substitute for Abdominal-B in the formation of the genitalia.
dc.description This work was supported by grants from the Ministerio de Educación y Ciencia (BMC2002-00300), the Comunidad Autónoma de Madrid (No. 08.1/0031/2001.1 and GR/SAL/0147/2004) and an Institutional Grant from the Fundación Ramón Areces.
dc.description Peer reviewed
dc.format 1868034 bytes
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dc.language eng
dc.publisher Elsevier
dc.relation http://dx.doi.org/10.1016/j.mod.2006.07.010
dc.rights openAccess
dc.subject Drosophila
dc.subject P-element
dc.subject Bithorax complex
dc.subject Gal4 lines
dc.subject Ubx
dc.subject Abd-B
dc.title A simple and efficient method to identify replacements of P-lacZ by P-Gal4 lines allows obtaining Gal4 insertions in the bithorax complex of Drosophila
dc.type Artículo


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