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Entropically-driven binding of mithramycin in the minor groove of C/G-rich DNA sequences

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dc.contributor Ministerio de Educación y Ciencia (España)
dc.contributor European Commission
dc.creator Barceló, Francisca
dc.creator Scotta, Claudia
dc.creator Ortiz-Lombardía, Miguel
dc.creator Méndez, Carmen
dc.creator Salas, José A.
dc.creator Portugal, José
dc.date 2008-05-30T11:43:18Z
dc.date 2008-05-30T11:43:18Z
dc.date 2007-03-16
dc.date.accessioned 2017-01-31T01:31:10Z
dc.date.available 2017-01-31T01:31:10Z
dc.identifier Nucleic Acids Res. 2007 April; 35(7): 2215–2226.
dc.identifier 0305-1048 (Print)
dc.identifier 1362-4962 (Online)
dc.identifier http://hdl.handle.net/10261/4691
dc.identifier 10.1093/nar/gkm037
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4691
dc.description Final full-text version available at: http://dx.doi.org/10.1093/nar/gkm037.-- Supplementary Data is available.
dc.description The antitumour antibiotic mithramycin A (MTA) is a DNA minor-groove binding ligand. It binds to C/G-rich tracts as a dimer that forms in the presence of divalent cations such as Mg2+. Differential scanning calorimetry, UV thermal denaturation, isothermal titration calorimetry and competition dialysis were used, together with computations of the hydrophobic free energy of binding, to determine the thermodynamic profile of MTA binding to DNA. The results were compared to those obtained in parallel using the structurally related mithramycin SK (MSK). The binding of MTA to salmon testes DNA determined by UV melting studies (Kobs = 1.2 (±0.3) x 10^5 M–1) is tighter than that of MSK (2.9 (±1.0) x 10^4 M–1) at 25°C. Competition dialysis studies showed a tighter MTA binding to both salmon testes DNA (42% C + G) and Micrococcus lysodeikticus DNA (72% C + G). The thermodynamic analysis of binding data at 25°C shows that the binding of MTA and MSK to DNA is entropically driven, dominated by the hydrophobic transfer of the antibiotics from solution to the DNA-binding site. Direct molecular recognition between MTA or MSK and DNA through hydrogen bonding and van der Waals contacts may also contribute significantly to complex formation.
dc.description Supported by a grant from the Spanish Ministry of Education and Science (SAF2005-00551) and the FEDER program of the European Community. This work was carried out within the framework of the Centre de Referencia en Biotecnologia of the Generalitat de Catalunya. Funding to pay the Open Access publication charge was provided by the Ministry of Education and Science and CSIC (Spain).
dc.description Peer reviewed
dc.format 219648 bytes
dc.format 1980270 bytes
dc.format 1352192 bytes
dc.format 84480 bytes
dc.format application/msword
dc.format application/pdf
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dc.language eng
dc.publisher Oxford University Press
dc.relation http://dx.doi.org/10.1093/nar/gkm037
dc.rights openAccess
dc.title Entropically-driven binding of mithramycin in the minor groove of C/G-rich DNA sequences
dc.type Artículo

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