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The regulated expression of chimeric tyrosine hydroxylase–insulin transcripts during early development

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dc.creator Hernández-Sánchez, Catalina
dc.creator Bártulos Encinas, Óscar
dc.creator Valenciano, Ana I.
dc.creator Mansilla, Alicia
dc.creator Pablo, Flora de
dc.date 2008-05-28T15:54:26Z
dc.date 2008-05-28T15:54:26Z
dc.date 2006-07-13
dc.date.accessioned 2017-01-31T01:28:54Z
dc.date.available 2017-01-31T01:28:54Z
dc.identifier Nucleic Acid Research 34(12):3465-3464(2006)
dc.identifier 0305-1048
dc.identifier PMCID: PMC1524912
dc.identifier http://hdl.handle.net/10261/4623
dc.identifier 10.1093/nar/gkl436
dc.identifier 1362-4962
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4623
dc.description Biological complexity does not appear to be simply correlated with gene number but rather other mechanisms contribute to the morphological and functional diversity across phyla. Such mechanisms regulate different transcriptional, translational and post-translational processes and include the recently identified transcription induced chimerism (TIC). We have found two novel chimeric transcripts in the chick and quail that result from the fusion of tyrosine hydroxylase (TH) and insulin into a single mature transcript. The th and insulin genes are located in tandem and they are generally transcribed independently. However, it appears that two chimeric transcripts containing exons from both the genes can also be produced in a regulated manner. The TH-INS1 and TH-INS2 chimeras differ in their insulin gene content, and they encode two novel isoforms of the TH protein with markedly reduced functionality when compared with the canonical TH. In addition, the TH-INS1 chimeric mRNA generates a small amount of insulin. We propose that TIC is an additional mechanism that can be employed to further regulate TH and insulin expression according to the specific needs of developing vertebrates.
dc.description These studies were financed by the grant BFU2004-2352 from the Spanish Ministry of Education and Science (MEC) and the Red de Grupos RGDM G03/212 from the ‘Instituto de Salud Carlos III’ (Spain) to F.P.; and by a grant from the ‘Comunidad de Madrid’ SAL/0647/2004 to C.H.-S. O.B. and A.M. were awarded a fellowship and C.H.-S. was a holder of a ‘Ramón y Cajal’ contract (all from the MEC, Spain). Funding to pay the Open Access publication charges for this article was provided by MEC (Spain).
dc.description Peer reviewed
dc.format 578991 bytes
dc.format 25600 bytes
dc.format application/pdf
dc.format application/vnd.ms-powerpoint
dc.language eng
dc.publisher Oxford University Press
dc.relation http://dx.doi.org/10.1093/nar/gkl436
dc.rights openAccess
dc.subject Gene-regulation
dc.subject Reading frames
dc.subject Fusion
dc.subject RNA
dc.subject Proinsulin
dc.subject Genome
dc.subject Catecholamines
dc.subject Identification
dc.subject Perspectives
dc.title The regulated expression of chimeric tyrosine hydroxylase–insulin transcripts during early development
dc.type Artículo

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