| dc.creator |
Yruela Guerrero, Inmaculada |
|
| dc.creator |
Alfonso Lozano, Miguel |
|
| dc.creator |
Barón Ayala, Matilde |
|
| dc.creator |
Picorel Castaño, Rafael |
|
| dc.date |
2008-05-19T14:59:40Z |
|
| dc.date |
2008-05-19T14:59:40Z |
|
| dc.date |
2000-12 |
|
| dc.date.accessioned |
2017-01-31T01:07:49Z |
|
| dc.date.available |
2017-01-31T01:07:49Z |
|
| dc.identifier |
Physiologia Plantarum 110 (4): 551-557 |
|
| dc.identifier |
0031-9317 |
|
| dc.identifier |
http://hdl.handle.net/10261/4350 |
|
| dc.identifier |
10.1111/j.1399-3054.2000.1100419.x |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4350 |
|
| dc.description |
The definitive version is available at:
http://www.blackwell-synergy.com/doi/full/10.1111/j.1399-3054.2000.1100419.x |
|
| dc.description |
We provide data from in vitro experiments on the polypeptide composition, photosynthetic electron transport and oxygen evolution activity of intact photosystem II (PSII) preparations under Cu(II) toxicity conditions. Low Cu(II) concentrations (Cu(II) per PSII reaction centre unit≤230) that caused around 50% inhibition of variable chlorophyll a fluorescence and oxygen evolution activity did not affect the polypeptide composition of PSII. However, the extrinsic proteins of 33, 24 and 17 kDa of the oxygen-evolving complex of PSII were removed when samples were treated with 300 μM CuCl2 (Cu(II) per PSII reaction centre unit=1 400). The LHCII antenna complex and D1 protein of the reaction centre of PSII were not affected even at these Cu(II) concentrations. The results indicated that the initial inhibition of the PSII electron transport and oxygen-evolving activity induced by the presence of toxic Cu(II) concentrations occurred before the damage of the oxygen-evolving complex. Indeed, more than 50% inhibition could be achieved in conditions where its protein composition and integrity was apparently preserved. |
|
| dc.description |
This work was supported by the Dirección General de Investigación Científica y Técnica (Grant PB98-1632). |
|
| dc.description |
Peer reviewed |
|
| dc.format |
366803 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Blackwell Publishing |
|
| dc.rights |
openAccess |
|
| dc.title |
Copper effect on the protein composition of photosystem II |
|
| dc.type |
Artículo |
|