| dc.creator |
Yruela Guerrero, Inmaculada |
|
| dc.creator |
Tomás Corruchaga, Raquel |
|
| dc.creator |
Sanjuán, M. L. |
|
| dc.creator |
Torrado Plou, Elena |
|
| dc.creator |
Aured Galve, María |
|
| dc.creator |
Picorel Castaño, Rafael |
|
| dc.date |
2008-05-12T12:32:52Z |
|
| dc.date |
2008-05-12T12:32:52Z |
|
| dc.date |
1998-11 |
|
| dc.date.accessioned |
2017-01-31T01:14:34Z |
|
| dc.date.available |
2017-01-31T01:14:34Z |
|
| dc.identifier |
Photochemistry and Photobiology 68(5): 725-737 |
|
| dc.identifier |
0031-8655 |
|
| dc.identifier |
http://hdl.handle.net/10261/4166 |
|
| dc.identifier |
10.1111/j.1751-1097.1998.tb02537.x |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4166 |
|
| dc.description |
The definitive version is available at http://www.blackwell-synergy.com/ |
|
| dc.description |
Different pigment extraction procedures and HPLC methods were tested to investigate the geometric configuration of the β-carotene in two forms of the photosystem II reaction center (Dl-D2-cytochrome (Cyt) b559) complex containing one and two β-carotene molecules per two pheophytin a. All the handling steps and HPLC analyses were done in darkness at room temperature and at 4°C. Two different pigment extracts were analyzed, a mixture of chlorophyll a, pheophytin a and 3-carotene, and the isolated p-carotene from that mixture. In both cases only the all-frans-β-carotene was detected. The chromatographic profiles were similar at both temperatures only differing in the retention times that were longer at 4°C. This result was independent of the concentration of photosynthetic starting material. Furthermore, no differences were observed between Dl-D2-Cyt b559 complexes with one and two p-carotene molecules per reaction center. The analysis of the β-carotene chromatographic peak indicated no 15-cis to all-fraws isom-erization occurred during the HPLC chromatography in our experimental conditions. Resonance Raman spectra were also recorded in the isolated Dl-D2-Cyt b559 complex at room and liquid nitrogen temperature with excitation at 514.5 nm from an Ar+ laser. Spectra of control preparations showed main bands at 1532, 1264, 1213, 1185, 1154 and 1003 cm−1 corresponding to the M-trans isomer and confirm previous results. The presence in the reaction center suspension of artificial electron acceptors such as silicomolybdate or 2,5-dibromo-3-methyl-6-iso-propyl-p-benzoquinone that are able to quench 3P680 did not modify the resonance Raman spectra of the native Dl-D2-Cyt b559 complex. The results suggest that no isomerization takes place during the laser irradiation. |
|
| dc.description |
This work was supported by the Dirección General de Investigación Científica y Técnica (Grant PB95-0219) to R.P. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
1468673 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Blackwell Publishing |
|
| dc.rights |
openAccess |
|
| dc.subject |
photosystem II |
|
| dc.subject |
reaction center |
|
| dc.subject |
beta-caroteno |
|
| dc.subject |
HPLC |
|
| dc.subject |
resonance raman |
|
| dc.title |
The configuration of beta-carotene in the photosystem II reaction center |
|
| dc.type |
Artículo |
|