| dc.creator |
Tous, Cristina |
|
| dc.creator |
Aguilera, Andrés |
|
| dc.date |
2008-05-08T15:46:25Z |
|
| dc.date |
2008-05-08T15:46:25Z |
|
| dc.date |
2007-08-24 |
|
| dc.date.accessioned |
2017-01-31T01:12:37Z |
|
| dc.date.available |
2017-01-31T01:12:37Z |
|
| dc.identifier |
Biochem Biophys Res Commun. 360(2): 428-432 (2007). |
|
| dc.identifier |
0006-291X |
|
| dc.identifier |
http://hdl.handle.net/10261/4096 |
|
| dc.identifier |
10.1016/j.bbrc.2007.06.098 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4096 |
|
| dc.description |
PMID: 17603014.-- Final version of the paper available at: http://www.sciencedirect.com/science/journal/0006291X |
|
| dc.description |
Transcription elongation causes a local change in DNA superhelicity. An excess of negative supercoiling may lead to opening of DNA strands that could allow formation of R-loops. In yeast, mutants of the THO complex are impaired in transcription elongation and this defect has been linked to co-transcriptional formation of R-loops, which could constitute roadblocks for RNA polymerases. In this study, we found that stably formed 300-nt long DNA–RNA hybrids in a negatively supercoiled transcription template reduced the efficiency of transcription elongation by half, providing a first experimental evidence that transcription elongation is impaired by R-loops in vitro. |
|
| dc.description |
This work was supported by grants of the Spanish Ministry of Science and Education (GEN2001-4707-C08-02 and SAF2003-00204) and Junta de Andalucía (CVI102). |
|
| dc.description |
Peer reviewed |
|
| dc.format |
22195 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Elsevier |
|
| dc.relation |
http://dx.doi.org/10.1016/j.bbrc.2007.06.098 |
|
| dc.rights |
closedAccess |
|
| dc.subject |
Saccharomyces cerevisiae |
|
| dc.subject |
R-loops |
|
| dc.subject |
Transcription elongation |
|
| dc.subject |
In vitro transcription |
|
| dc.subject |
RNA polymerase II |
|
| dc.subject |
DNA–RNA hybrid |
|
| dc.subject |
RNA |
|
| dc.title |
Impairment of transcription elongation by R-loops in vitro |
|
| dc.type |
Artículo |
|