| dc.creator |
García-Rubio, María L. |
|
| dc.creator |
Chávez, Sebastián |
|
| dc.creator |
Huertas Sánchez, Pablo |
|
| dc.creator |
Tous, Cristina |
|
| dc.creator |
Jimeno, Sonia |
|
| dc.creator |
Luna, Rosa |
|
| dc.creator |
Aguilera, Andrés |
|
| dc.date |
2008-05-08T15:29:56Z |
|
| dc.date |
2008-05-08T15:29:56Z |
|
| dc.date |
2008-02 |
|
| dc.date.accessioned |
2017-01-31T01:12:34Z |
|
| dc.date.available |
2017-01-31T01:12:34Z |
|
| dc.identifier |
Molecular Genetics and Genomics 279(2): 123–132 (2008) |
|
| dc.identifier |
1617-4615 |
|
| dc.identifier |
http://hdl.handle.net/10261/4095 |
|
| dc.identifier |
10.1007/s00438-007-0301-6 |
|
| dc.identifier |
1617-4623 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4095 |
|
| dc.description |
PMID: 17960421.-- Final full-text version available at: http://dx.doi.org/10.1007/s00438-007-0301-6 (www.springerlink.com) |
|
| dc.description |
THO/TREX is a conserved nuclear complex that functions in mRNP biogenesis and plays a role in preventing the transcription-associated genetic instability. THO is composed of Tho2, Hpr1, Mft1 and Thp2 subunits, which associate with the Sub2-Yra1 export factors and Tex1 to form the TREX complex. To compare the functional relevance of the different THO/TREX subunits, we determined the effect of their null mutations on mRNA accumulation and recombination. Unexpectedly, we noticed that a full deletion of HPR1, hpr1DeltaK, conferred stronger hyper-recombination phenotype and gene expression defects than did hpr1DeltaH, the allele encoding a C-terminal truncated protein which was used in most previous studies. We show that tho2Delta and, to a lesser extent, hpr1DeltaK are the THO mutations with the highest impact on all phenotypes, and that sub2Delta shows a similar transcription-dependent hyper-recombination phenotype and in vivo transcription impairment as hpr1DeltaK and tho2Delta. Recombination and transcription analyses indicate that THO/TREX mutants share a moderate but significant effect on gene conversion and ectopic recombination, as well as transcription impairment of even short and low GC-content genes. Our data provide new information on the relevance of these proteins in mRNP biogenesis and in the maintenance of genomic integrity. |
|
| dc.description |
This work was supported by the Ministry of Science and Education of Spain (grants SAF2003–00204 and BMC2006–05260) and Junta de Andalucía (CVI-102). |
|
| dc.description |
Peer reviewed |
|
| dc.format |
22195 bytes |
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| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Springer |
|
| dc.relation |
http://dx.doi.org/10.1007/s00438-007-0301-6 |
|
| dc.rights |
closedAccess |
|
| dc.subject |
THO complex |
|
| dc.subject |
Sub2 |
|
| dc.subject |
Genetic instability |
|
| dc.subject |
mRNP biogenesis |
|
| dc.subject |
Transcription |
|
| dc.subject |
Transcription-associated recombination |
|
| dc.title |
Different physiological relevance of yeast THO/TREX subunits in gene expression and genome integrity |
|
| dc.type |
Artículo |
|