أعرض تسجيلة المادة بشكل مبسط
| dc.creator |
Gaillard, Hélène |
|
| dc.creator |
Wellinger, Ralf Erik |
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| dc.creator |
Aguilera, Andrés |
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| dc.date |
2008-05-08T11:51:42Z |
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| dc.date |
2008-05-08T11:51:42Z |
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| dc.date |
2007-06 |
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| dc.date.accessioned |
2017-01-31T01:12:09Z |
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| dc.date.available |
2017-01-31T01:12:09Z |
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| dc.identifier |
Nucleic Acids Res. 35(12): 3893–3906 (2007). |
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| dc.identifier |
0305-1048 |
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| dc.identifier |
http://hdl.handle.net/10261/4090 |
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| dc.identifier |
10.1093/nar/gkm373 |
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| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4090 |
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| dc.description |
PMCID: PMC1919492 |
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| dc.description |
Although DNA repair is faster in the transcribed strand of active genes, little is known about the possible contribution of mRNP biogenesis and export in transcription-coupled repair (TCR). Interestingly, mutants of THO, a transcription complex involved in maintenance of genome integrity, mRNP biogenesis and export, were recently found to be deficient in nucleotide excision repair. In this study we show by molecular DNA repair analysis, that Sub2-Yra1 and Thp1-Sac3, two main mRNA export complexes, are required for efficient TCR in yeast. Careful analysis revealed that THO mutants are also specifically affected in TCR. Ribozyme-mediated mRNA self-cleavage between two hot spots for UV damage showed that efficient TCR does not depend on the nascent mRNA, neither in wild-type nor in mutant cells. Along with severe UV damage-dependent loss in processivity, RNAPII was found binding to chromatin upon UV irradiation in THO mutants, suggesting that RNAPII remains stalled at DNA lesions. Furthermore, Def1, a factor responsible for the degradation of stalled RNAPII, appears essential for the viability of THO mutants subjected to DNA damage. Our results indicate that RNAPII is not proficient for TCR in mRNP biogenesis and export mutants, opening new perspectives on our knowledge of TCR in eukaryotic cells. |
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| dc.description |
Research was funded by grants from the Spanish Ministry of Science and Education (SAF2003-00204 and BFU2006-05260) and from the Junta de Andalucía (CVI102). HG
was granted by the Swiss National Science Foundation (PBEZA-100700 and PA00A-105027) and the Novartis Foundation. Funding to pay the Open Access publication charges for this article was provided by Spanish Ministry of Science and Education grant BFU2006-05260. |
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| dc.description |
Peer reviewed |
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| dc.format |
6478775 bytes |
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| dc.format |
application/pdf |
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| dc.language |
eng |
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| dc.publisher |
Oxford University Press |
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| dc.rights |
openAccess |
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| dc.subject |
DNA repair |
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| dc.subject |
Transcription-Coupled Repair (TCR) |
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| dc.subject |
THO mutants |
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| dc.title |
A new connection of mRNP biogenesis and export with transcription-coupled repair |
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| dc.type |
Artículo |
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