| dc.creator |
Abdu, Uri |
|
| dc.creator |
González-Reyes, Acaimo |
|
| dc.creator |
Ghabrial, Amin |
|
| dc.creator |
Schüpbach, Trudi |
|
| dc.date |
2008-05-06T16:09:41Z |
|
| dc.date |
2008-05-06T16:09:41Z |
|
| dc.date |
2003-09 |
|
| dc.date.accessioned |
2017-01-31T01:11:02Z |
|
| dc.date.available |
2017-01-31T01:11:02Z |
|
| dc.identifier |
Genetics 165: 197–204 (September 2003). |
|
| dc.identifier |
http://www.genetics.org/cgi/content/abstract/165/1/197 |
|
| dc.identifier |
0016-6731 |
|
| dc.identifier |
http://hdl.handle.net/10261/4048 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4048 |
|
| dc.description |
Sequence data from this article have been deposited with the Gen-Bank data libraries under accession no. AY257540. |
|
| dc.description |
In Drosophila, mutations in double-strand DNA break (DSB) repair enzymes, such as spn-B, activate a meiotic checkpoint leading to dorsal-ventral patterning defects in the egg and an abnormal appearance of the oocyte nucleus. Mutations in spn-D cause an array of ovarian phenotypes similar to spn-B. We have cloned the spn-D locus and found that it encodes a protein of 271 amino acids that shows significant homology to the human RAD51C protein. In mammals the spn-B and spn-D homologs, XRCC3 and RAD51C, play a role in genomic stability in somatic cells. To test for a similar role for spn-B and spn-D in double-strand DNA repair in mitotic cells, we analyzed the sensitivity of single and double mutants to DSBs induced by exposure to X rays and MMS. We found that neither singly mutant nor doubly mutant animals were significantly sensitized to MMS or X rays. These results suggest that spn-B and spn-D act in meiotic recombination but not in repair of DSBs in somatic cells. As there is no apparent ortholog of the meiosis-specific DMC1 gene in the Drosophila genome, and given their meiosis-specific requirement, we suggest that spn-B and spn-D may have a function comparable to DMC1. |
|
| dc.description |
This work was supported by the PHS Grant PO1CA41086 and by the Howard Hughes Medical
Institute. A.G.-R. acknowledges the financial support of the Spanish Ministerio de Ciencia y Tecnología (grant nos. PM99-0107 and BMC2001-4822-E), the Junta de Andalucía (CVI 724), and the EMBO Young Investigator Programme. |
|
| dc.description |
Peer reviewed |
|
| dc.format |
229365 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Genetics Society of America |
|
| dc.rights |
openAccess |
|
| dc.subject |
Mutations |
|
| dc.subject |
Drosophila |
|
| dc.subject |
DNA breaks |
|
| dc.subject |
Repair enzymes |
|
| dc.subject |
Recombination |
|
| dc.subject |
Meiosis |
|
| dc.title |
The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosis |
|
| dc.type |
Artículo |
|