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Drosophila mus301/spindle-C Encodes a Helicase With an Essential Role in Double-Strand DNA Break Repair and Meiotic Progression

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dc.creator McCaffrey, Ruth
dc.creator St Johnston, Daniel
dc.creator González-Reyes, Acaimo
dc.date 2008-05-06T15:58:40Z
dc.date 2008-05-06T15:58:40Z
dc.date 2006-11
dc.date.accessioned 2017-01-31T01:11:00Z
dc.date.available 2017-01-31T01:11:00Z
dc.identifier Genetics. 2006 November; 174(3): 1273–1285.
dc.identifier http://dx.doi.org/10.1534/genetics.106.058289
dc.identifier 0016-6731
dc.identifier http://hdl.handle.net/10261/4047
dc.identifier 10.1534/genetics.106.058289
dc.identifier.uri http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4047
dc.description PMCID: PMC1667076
dc.description mus301 was identified independently in two genetic screens, one for mutants hypersensitive to chemical mutagens and another for maternal mutants with eggshell defects. mus301 is required for the proper specification of the oocyte and for progression through meiosis in the Drosophila ovary. We have cloned mus301 and show that it is a member of the Mus308 subfamily of ATP-dependent helicases and the closest homolog of human and mouse HEL308. Functional analyses demonstrate that Mus301 is involved in chromosome segregation in meiosis and in the repair of double-strand-DNA breaks in both meiotic and mitotic cells. Most of the oogenesis defects of mus301 mutants are suppressed by mutants in the checkpoint kinase Mei41 and in MeiW68, the Spo11 homolog that is thought to generate the dsDNA breaks that initiate recombination, indicating that these phenotypes are caused by activation of the DNA damage checkpoint in response to unrepaired Mei-W68-induced dsDNA breaks. However, neither mei-W68 nor mei-41 rescue the defects in oocyte specification of mus301 mutants, suggesting that this helicase has another function in oocyte selection that is independent from its role in meiotic recombination.
dc.description Financial support from the European Community Marie Curie Programme to R.M.; from the Medical Research Council (UK), the Spanish Ministerio de Ciencia y Tecnología (BMC2003-01512), and the Junta de Andalucía (CVI-280) to A.G.-R.; and from a Wellcome Trust (UK) Principal Research Fellowship to D.StJ. is acknowledged.
dc.description Peer reviewed
dc.format 2686103 bytes
dc.format application/pdf
dc.language eng
dc.publisher Genetics Society of America
dc.rights openAccess
dc.subject mus301
dc.subject Drosophila
dc.subject Chromosome segregation
dc.subject Meiosis
dc.subject DNA breaks
dc.subject Recombination
dc.subject Oogenesis
dc.title Drosophila mus301/spindle-C Encodes a Helicase With an Essential Role in Double-Strand DNA Break Repair and Meiotic Progression
dc.type Artículo


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